Identification and purification of a soluble region in the breast cancer susceptibility protein BRCA2

被引：2

作者：

Finch, D ^{[1
]}

Webb, M ^{[1
]}

机构：

[1] Univ Manchester, Dept Med Genet, Ctr Mol Med, Manchester M13 9PT, Lancs, England

来源：

PROTEIN EXPRESSION AND PURIFICATION | 2005年 / 40卷 / 01期

关键词：

BRCA2; limited proteolysis; domain;

D O I：

10.1016/j.pep.2004.10.025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The BRCA2 gene encodes a large multidomain protein of 3418 residues. Studies to elucidate the mechanisms by which BRCA2 prevents tumour formation have been largely restricted by the size of the protein. Based on secondary structure predictions we have cloned regions across the BRCA2 gene and determined the solubility of the proteins they encode. The fragment consisting of amino acids 290-456 BRCA2 was found predominantly in the soluble portion of the cell lysate and was purified by ion exchange and nickel-NTA affinity chromatography. CD spectroscopy revealed secondary structure elements consistent with a folded peptide and limited proteolysis was used to identify a potential novel domain. (c) 2004 Elsevier Inc. All rights reserved.

引用

页码：177 / 182

页数：6

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