Aptamer-based biosensors for the rapid visual detection of flu viruses

被引：44

作者：

Le, T. T. ^{[1
,2
]}

Adamiak, B. ^{[3
]}

Benton, D. J. ^{[4
]}

Johnson, C. J. ^{[1
,2
]}

Sharma, S. ^{[1
,2
]}

Fenton, R. ^{[5
]}

McCauley, J. W. ^{[4
]}

Iqbal, M. ^{[3
]}

Cass, A. E. G. ^{[1
,2
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England

[2] Univ London Imperial Coll Sci Technol & Med, Inst Biomed Engn, London SW7 2AZ, England

[3] Pirbright Inst, Newbury, Berks, England

[4] MRC, Natl Inst Med Res, Div Virol, London W1N 4AL, England

[5] Iceni Labs Ltd, London, England

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 98期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

INFLUENZA-A VIRUS; RNA APTAMER; ENZYME-IMMUNOASSAY; VIRAL CULTURE; DNA APTAMER; HEMAGGLUTININ; NANOSTRUCTURES; NANOPARTICLES; ANTIBODIES; SEPARATION;

D O I：

10.1039/c4cc07888h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

RNA aptamers showing affinity and specificity for different strains of human influenza virus were assembled onto gold nanoparticles that subsequently formed a gold nanoshell (AuNS) around the viral envelope. These shells could be visualised by transmission electron microscopy (TEM). Changes in size and structure of the AuNS coated virus can be used to detect the viruses. We show that sedimentation with a low cost centrifuge and visual determination can detect 3 x 10(8) viral particles.

引用

页码：15533 / 15536

页数：4

共 43 条

[1] Separation of Nanoparticles in Aqueous Multiphase Systems through Centrifugation [J].

Akbulut, Ozge ;

Mace, Charles R. ;

Martinez, Ramses V. ;

Kumar, Ashok A. ;

Nie, Zhihong ;

Patton, Matthew R. ;

Whitesides, George M. .

NANO LETTERS, 2012, 12 (08) :4060-4064

[2]

[Anonymous], NAT REV DRUG DISCOV

[3] Characterization of Pandemic Influenza A (H1N1) Virus Hemagglutinin Specific Polyclonal Antibodies for Biosensor Applications [J].

Athmaram, T. N. ;

Saraswat, Shweta ;

Sikarwar, Bhavna ;

Verma, Shailendra Kumar ;

Singh, Anil K. ;

Boopathi, M. .

JOURNAL OF MEDICAL VIROLOGY, 2014, 86 (03) :363-371

[4] Determination of nanoparticle size distribution together with density or molecular weight by 2D analytical ultracentrifugation [J].

Carney, Randy P. ;

Kim, Jin Young ;

Qian, Huifeng ;

Jin, Rongchao ;

Mehenni, Hakim ;

Stellacci, Francesco ;

Bakr, Osman M. .

NATURE COMMUNICATIONS, 2011, 2

[5] Comparison of the directigen flu A+B membrane enzyme immunoassay with viral culture for rapid detection of influenza A and B viruses in respiratory specimens [J].

Cazacu, AC ;

Chung, SE ;

Greer, J ;

Demmler, GJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (08) :3707-3710

[6] Comparison of lateral-flow immunoassay and enzyme immunoassay with viral culture for rapid detection of influenza virus in nasal wash specimens from children [J].

Cazacu, AC ;

Greer, J ;

Taherivand, M ;

Demmler, GJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (05) :2132-2134

[7] Recent H3N2 Influenza Virus Clinical Isolates Rapidly Acquire Hemagglutinin or Neuraminidase Mutations When Propagated for Antigenic Analyses [J].

Chambers, Benjamin S. ;

Li, Yang ;

Hodinka, Richard L. ;

Hensley, Scott E. .

JOURNAL OF VIROLOGY, 2014, 88 (18) :10986-10989

[8] Evaluation of the Directigen FluA+B test for rapid diagnosis of influenza virus type A and B infections [J].

Chan, KH ;

Maldeis, N ;

Pope, W ;

Yup, A ;

Ozinskas, A ;

Gill, J ;

Seto, WH ;

Shortridge, KF ;

Peiris, JSM .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (05) :1675-1680

[9] Applications of Aptamers as Sensors [J].

Cho, Eun Jeong ;

Lee, Joo-Woon ;

Ellington, Andrew D. .

ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, 2009, 2 :241-264

[10] High Efficiency Acetylcholinesterase Immobilization on DNA Aptamer Modified Surfaces [J].

Chumphukam, Orada ;

Le, Thao T. ;

Cass, Anthony E. G. .

MOLECULES, 2014, 19 (04) :4986-4996

← 1 2 3 4 5 →