Characterization of targeting domains by sequence analysis: glycogen-binding domains in protein phosphatases

The prediction of functional features from sequence data can powerfully complement experimental analyses. Domains are delineated in a first step. Subsequent analysis can provide functional information that even may include precise structural details. This contribution describes the way in which sequence analysis has used multiple alignment, blast and reverse blast searches, and other, more sophisticated techniques to produce a detailed picture of the glycogen-targeting domain in the glycogen-binding subunit of the protein phosphatase 1 (PP1) family that includes its three-dimensional fold.