Twelve-gel slide format optimised for comet assay and fluorescent in situ hybridisation

被引：76

作者：

Shaposhnikov, Sergey ^{[1
]}

Azqueta, Amaya ^{[1
]}

Henriksson, Sara ^{[2
]}

Meier, Silja ^{[1
,3
]}

Gaivao, Isabel ^{[4
]}

Huskisson, Neville H. ^{[5
]}

Smart, Andrew ^{[5
]}

Brunborg, Gunnar ^{[3
]}

Nilsson, Mats ^{[2
]}

Collins, Andrew R. ^{[1
]}

机构：

[1] Univ Oslo, Dept Nutr, N-0316 Oslo, Norway

[2] Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol, SE-75185 Uppsala, Sweden

[3] Norwegian Inst Publ Hlth, N-0403 Oslo, Norway

[4] Univ Tras os Montes & Alto Douro, Genet & Biotechnol Dept, P-5000 Vila Real, Portugal

[5] Severn Biotech Ltd, Unit 2, Kidderminster DY11 6TJ, Worcs, England

来源：

TOXICOLOGY LETTERS | 2010年 / 195卷 / 01期

关键词：

Comet assay; Fluorescent in situ hybridisation; High throughput method; DNA; DAMAGE;

D O I：

10.1016/j.toxlet.2010.02.017

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 [卫生毒理学];

摘要：

The comet assay is widely used to measure DNA damage and repair in basic research, genotoxicity testing and human biomonitoring. The conventional format has 1 or 2 gels on a microscope slide, 1 sample per slide. To increase throughput, we have designed and tested a system with 12 smaller gels on one slide, allowing incubation of individual gels with different reagents or enzymes. Thus several times more samples can be analysed with one electrophoresis run, and fewer cells and smaller volumes of test solutions are required. Applications of the modified method include treatment with genotoxic agents at different concentrations; simultaneous analysis of different lesions using a range of enzymes; analysis of cell extracts for DNA repair activity; and fluorescent in situ hybridisation (FISH) to comet DNA with specific labelled probes. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

引用

页码：31 / 34

页数：4

共 7 条

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The comet assay for DNA damage and repair - Principles, applications, and limitations [J].