Effects of long-term treatment with bovine somatotropin on follicular dynamics and subsequent oocyte and blastocyst yield in an OPU-IVF program

被引:31
作者
Bols, PEJ
Ysebaert, MT
Lein, A
Coryn, M
Van Soom, A
de Kruif, A
机构
[1] State Univ Ghent, Fac Vet Med, Dept Obstet Reprod & Herd Hlth, B-9000 Ghent, Belgium
[2] State Univ Ghent, Fac Vet Med, Dept Physiol Biochem & Biometr, B-9000 Ghent, Belgium
关键词
OPU; bovine somatotropin; oocyte quality; IVF;
D O I
10.1016/S0093-691X(98)00047-8
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Fourteen Holstein-Friesian heifers between 15 and 22 months of age with normal reproductive tracts were used in an experimental set up to investigate the effect of a long term rBST-treatment on the follicular population prior to transvaginal ovum pick-up (OPU). The estrous cycle of the animals was synchronized by means of a double injection of 2 ml cloprostenol 11 days apart. The heifers were divided in 2 equal groups (n=7) in which the animals had the same average body weight, one group receiving a weekly subcutaneous injection of 640 mg recombinantly derived bovine somatotropin (rBST) on Mondays (rBST-treatment group) and a control group, being injected with 10 ml of saline. Heifers in both groups were submitted to OPU twice a week on Mondays and Thursdays using a 5 Mhz transducer and a disposable, 55 mm long 20-g short bevelled needle at a vacuum pressure corresponding to approximately 13 ml water/min The experimental period lasted for 10 weeks (April to June), each animal receiving a total of 10 injections and being submitted to OPU for 20 times. Oocytes were subsequently matured and cultured in a separate drop per cow following conventional IVF procedures. A blood sample was taken on heparin immediately after each OPU session, for determination of blood progesterone concentrations to assess the influence of treatment and OPU procedure on the cow's estrous cycle. Although results show a significant increase in the total number of follicles and medium sized follicles in the rBST-treated group, no statistically significant different number of retrieved oocytes between the rBST-treated and nontreated group could be detected. The average number of retrieved oocytes per session per cow was comparable, being 6.4 for the treated and 6.0 for the control group. Additionally, the average number of blastocysts per cow per session did not differ significantly between groups, being 1.41 in the rBST-treated group and 1.53 in the control group. The number of cultured oocytes which developed to the blastocyst stage was 22% in the rBST-treated group, which was not significantly different from 25% in the control group. Repeated OPU appeared to induce a certain degree of acyclicity in both treated and nontreated animals. (C) 1998 by Elsevier Science Inc.
引用
收藏
页码:983 / 995
页数:13
相关论文
共 56 条
[1]   RECENT ADVANCES IN SUPEROVULATION OF CATTLE [J].
ARMSTRONG, DT .
THERIOGENOLOGY, 1993, 39 (01) :7-24
[2]  
BALTUSSEN RMW, 1992, P 12 INT C AN REPR, V1, P129
[3]   EXOGENOUS CONTROL OF FOLLICULAR WAVE EMERGENCE IN CATTLE [J].
BO, GA ;
ADAMS, GP ;
PIERSON, RA ;
MAPLETOFT, RJ .
THERIOGENOLOGY, 1995, 43 (01) :31-40
[4]   ALTERNATIVE GONADOTROPINS FOR SUPEROVULATION IN CATTLE [J].
BOLAND, MP ;
GOULDING, D ;
ROCHE, JF .
THERIOGENOLOGY, 1991, 35 (01) :5-17
[5]  
Bols P. E. J., 1997, Theriogenology, V47, P315, DOI 10.1016/S0093-691X(97)82442-9
[6]  
Bols P. E. J., 1996, Theriogenology, V45, P359, DOI 10.1016/0093-691X(96)84832-1
[7]   TRANSVAGINAL OVUM PICK-UP (OPU) IN THE COW - A NEW DISPOSABLE NEEDLE GUIDANCE-SYSTEM [J].
BOLS, PEJ ;
VANDENHEEDE, JMM ;
VANSOOM, A ;
DEKRUIF, A .
THERIOGENOLOGY, 1995, 43 (03) :677-687
[8]   Effects of needle tip bevel and aspiration procedure on the morphology and developmental capacity of bovine compact cumulus oocyte complexes [J].
Bols, PEJ ;
Ysebaert, MT ;
VanSoom, A ;
deKruif, A .
THERIOGENOLOGY, 1997, 47 (06) :1221-1236
[9]   Effects of aspiration vacuum and needle diameter on cumulus oocyte complex morphology and developmental capacity of bovine oocytes [J].
Bols, PEJ ;
VanSoom, A ;
Ysebaert, MT ;
Vandenheede, JMM ;
deKruif, A .
THERIOGENOLOGY, 1996, 45 (05) :1001-1014
[10]  
Britt J. H., 1992, Proceedings of the Twenty Fourth Annual Convention American Association of Bovine Practitioners September 18-21, 1991 Orlando, Florida., P39