Type III secretion homologs are present in Brucella melitensis, B-ovis, and B-suis biovars 1, 2, and 3

被引:10
作者
Abdallah, AI
Commander, NJ
Woodward, MJ
Spencer, S
Hart, CA
Winstanley, C
机构
[1] Univ Liverpool, Dept Med Microbiol & Genitourinary Med, Liverpool L69 3GA, Merseyside, England
[2] Vet Labs Agcy, Dept Bacterial Dis, Weybridge KT15 3NB, Surrey, England
[3] Vet Med Directorate, Addlestone KT15 3NB, Surrey, England
关键词
D O I
10.1007/s00284-002-3789-3
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Protein sequences from characterized type III secretion (TTS) systems were used as probes in silico to identify several TTS gene homologs in the genome sequence of Brucella suis biovar 1 strain 1330. Four of the genes, named flhB, fliP, fliR, and fliF on the basis of greatest homologies to known flagellar apparatus proteins, were targeted in PCR and hybridization assays to determine their distribution among other Brucella nomen species and biovars. The results indicated that flhB, fliP, fliR and fliF are present in Brucella melitensis, Brucella ovis, and Brucella suis biovars 1, 2 and 3. Similar homologos have been reported previously in Brucella abortus. Using RT-PCR assays, we were unable to detect any expression of these genes. It is not yet known whether the genes are the cryptic remnants of a flagellar system or are actively involved in a process contributing to pathogenicity or previously undetected motility, but they are distributed widely in Brucella and merit further study to determine their role.
引用
收藏
页码:241 / 245
页数:5
相关论文
共 18 条
[1]  
CORBEL MJ, 1983, METHODS IDENTIFICATI
[2]  
Davis D. S., 1990, Animal brucellosis., P321
[3]   Brucella abortus genes identified following constitutive growth and macrophage infection [J].
Eskra, L ;
Canavessi, A ;
Carey, M ;
Splitter, G .
INFECTION AND IMMUNITY, 2001, 69 (12) :7736-7742
[4]   EXPRESSION OF FLAGELLA AND MOTILITY BY SHIGELLA [J].
GIRON, JA .
MOLECULAR MICROBIOLOGY, 1995, 18 (01) :63-75
[5]   CLONING AND EXPRESSION IN PSEUDOMONAS-AERUGINOSA OF A GENE INVOLVED IN THE PRODUCTION OF ALGINATE [J].
GOLDBERG, JB ;
OHMAN, DE .
JOURNAL OF BACTERIOLOGY, 1984, 158 (03) :1115-1121
[6]  
Halling S M, 1998, Microb Comp Genomics, V3, P21, DOI 10.1089/omi.1.1998.3.21
[7]   Type III protein secretion systems in bacterial pathogens of animals and plants [J].
Hueck, CJ .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (02) :379-+
[8]  
LEIGH AF, 1993, FEMS MICROBIOL LETT, V111, P225
[9]  
Nicoletti P, 1980, Adv Vet Sci Comp Med, V24, P69
[10]   Gene discovery through genomic sequencing of Brucella abortus [J].
Sánchez, DO ;
Zandomeni, RO ;
Cravero, S ;
Verdún, RE ;
Pierrou, E ;
Faccio, P ;
Diaz, G ;
Lanzavecchia, S ;
Agüero, F ;
Frasch, ACC ;
Andersson, SGE ;
Rossetti, OL ;
Grau, O ;
Ugalde, RA .
INFECTION AND IMMUNITY, 2001, 69 (02) :865-868