Cardiac myocyte force development during differentiation and maturation

被引：76

作者：

Jacot, Jeffrey G. ^{[4
,5
]}

Kita-Matsuo, Hiroko ^{[3
]}

Wei, Karen A. ^{[1
,3
]}

Chen, H. S. Vincent ^{[2
,3
]}

Omens, Jeffrey H. ^{[1
,2
]}

Mercola, Mark ^{[3
]}

McCulloch, Andrew D. ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA

[3] Burnham Inst Med Res, La Jolla, CA USA

[4] Texas Childrens Hosp, Baylor Coll Med, Div Congenital Heart Surg, Houston, TX 77030 USA

[5] Rice Univ, Dept Bioengn, Houston, TX USA

来源：

ANALYSIS OF CARDIAC DEVELOPMENT: FROM EMBRYO TO OLD AGE | 2010年 / 1188卷

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

cardiac myocyte; mechanotransduction; substrate stiffness; gel; stem cell; differentiation; EMBRYONIC STEM-CELLS; SUBSTRATE MECHANICS; STIFFNESS AFFECTS; CYCLIC STRAIN; GEL STIFFNESS; CHEMISTRY; MATRIX; ADHESION; CREATION;

D O I：

10.1111/j.1749-6632.2009.05091.x

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

The maturation of cardiac myocytes during the immediate prenatal period coincides with changes in the mechanical properties of the extracellular matrix. We investigated the effects of extracellular stiffness on cardiomyocyte maturation in neonatal rat ventricular myocytes grown on collagen-coated gels. Cells on 10-kPa substrates developed aligned sarcomeres, while cells on stiffer substrates had unaligned sarcomeres and stress fibers. Cells generated greater mechanical force on gels with stiffness similar to that of the native myocardium than on stiffer or softer substrates. To investigate the differentiation of myocyte progenitors, we used clonal expansion of engineered human embryonic stem cells. Puromycin-selected cardiomyocytes exhibited a gene expression profile similar to that of adult human cardiomyocytes and generated force and action potentials consistent with normal fetal cardiomyocytes. These results suggest that extracellular stiffness significantly affects maturation and differentiation of immature ventricular myocytes.

引用

页码：121 / 127

页数：7

共 30 条

[1]

Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures [J].