Determination of Ro 48-3656 in rat plasma by reversed-phase high-performance liquid chromatography -: Comparison of 1.5-μm nonporous silica to 3.5-μm porous silica analytical columns

We describe a method for measuring Ro 48-3656 in EDTA rat plasma by neutral pH, reversed-phase high-performance liquid chromatography using a 1.5-mu m nonporous silica, C-18 analytical column and UV absorbance detection to support pharmacokinetic studies. We also describe a comparison of the 1.5-mu m nonporous silica C-18 column versus 3.5-mu m porous silica C-18 columns. The final method using the 1.5-mu m nonporous silica column demonstrated good precision (of both quantification and retention time), accuracy and recovery, linearity of dilution and limit of quantification (40 ng/ml Ro 48-3656 using a 20 mu l injection). Samples of neat EDTA rat plasma were prepared by ultrafiltration followed by direct injection onto the HPLC column. (C) 1997 Elsevier Science B.V.