Increased nuclear factor-κB and angiotensinogen gene expression in postransplant recurrent focal segmental glomerulosclerosis

被引:13
作者
Schachter, AD
Strehlau, J
Zurakowski, D
Vasconcellos, L
Kim, YS
Zheng, XX
Gunshin, T
Overstreet, SL
Benfield, MR
Tejani, H
Harmon, WE
Herrin, JT
Strom, TB
机构
[1] Childrens Hosp, Div Nephrol, Boston, MA 02115 USA
[2] Childrens Hosp, Dept Biostat, Boston, MA 02115 USA
[3] Harvard Univ, Sch Med, Dept Pediat, Boston, MA 02115 USA
[4] Harvard Univ, Sch Med, Dept Med, Boston, MA 02115 USA
[5] Beth Israel Deaconess Med Ctr, Div Immunol, Boston, MA 02215 USA
[6] Univ Alabama, Dept Pediat, Birmingham, AL 35233 USA
[7] N Amer Pediat Renal Transplant Cooperat Study, Clin Coordinating Ctr, Hawthorne, NY 10532 USA
关键词
D O I
10.1097/00007890-200010150-00021
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In an attempt to identify potential markers of steroid-resistance in focal segmental glomerulosclerosis (FSGS) we evaluated intra-graft gene expression of I kappaB alpha, nuclear factor-kappaB (NF-kappaB), and angiotensinogen in 60 biopsies from 27 pediatric renal transplant recipients. Intra-graft NF-kappaB expression was significantly elevated in recurrent FSGS (R-FSGS) (218.3 + 55.6 ag/fg versus NON-FSGS 121.1 + 19.9, P=0.04) but not in acute rejection. NF-kappaB:I kappaB alpha ratios were higher in cadaveric donor versus living related donor recipients (15.7 + 2.8 vs. 8.8 + 1.3, respectively, P=0.015), and in African-American versus Caucasian recipients (15.6 + 2.9 vs. 9.1 + 1.3, respectively, P=0.03). Intra-graft angiotensinogen gene expression was significantly elevated in R-FSGS (30.5 + 8.8 ag/fg R-FSGS vs. 16.0 + 4.7 NON-FSGS, P=0.009), We conclude that increased NF-kappaB and angiotensinogen gene expression are associated with R-FSGS. Increased NF-kappaB:I kappaB alpha ratios are associated with cadaveric donor recipients and African-American race.
引用
收藏
页码:1107 / 1110
页数:4
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