In vitro genotoxicity testing of ARASCO® and DHASCO® oils

被引：24

作者：

Arterburn, LM

Boswell, KD

Lawlor, T

Cifone, MA

Murli, H

Kyle, DJ

机构：

[1] Martek Biosci Corp, Columbia, MD 21043 USA

[2] Covance Labs, Vienna, VA USA

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2000年 / 38卷 / 11期

关键词：

docosahexaenoic acid (DHA); arachidonic acid (ARA); single-cell oils; genotoxicity; Crypthecodinium cohnii; Mortierella alpina;

D O I：

10.1016/S0278-6915(00)00085-5

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

ARASCO(R) and DHASCO(R) oils are microbially-derived triglycerides rich in arachidonic (20:4n-6) and docosahexaenoic (22:6n-3) acids, respectively. Both oils mere tested for mutagenic activity in three different in vitro mutagenesis assays. All assays were conducted with and without metabolic activation. Neither ARASCO nor DHASCO oil was mutagenic in the Ames reverse mutation assay using five different Salmonella histidine auxotroph tester strains, nor were the oils mutagenic in the mouse lymphoma TK+/- forward mutation assay. The oils showed no clastogenic activity in chromosomal aberration assays performed with Chinese hamster ovary cells. Based on these assays, neither ARASCO nor DHASCO oils appear to have any genotoxic potential. (C) 2000 Elsevier Science Ltd. All rights reserved.

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页码：971 / 976

页数：6

相关论文

共 20 条

[1] NEURODEVELOPMENTAL QUOTIENT OF HEALTHY TERM INFANTS AT 4 MONTHS AND FEEDING PRACTICE - THE ROLE OF LONG-CHAIN POLYUNSATURATED FATTY-ACIDS [J].

AGOSTONI, C ;

TROJAN, S ;

BELLU, R ;

GIOVANNINI, M .

PEDIATRIC RESEARCH, 1995, 38 (02) :262-266

[2] POINT MUTATIONS AT THE THYMIDINE KINASE LOCUS IN L5178Y MOUSE LYMPHOMA-CELLS .2. TEST VALIDATION AND INTERPRETATION [J].

AMACHER, DE ;

PAILLET, SC ;

TURNER, GN ;

RAY, VA ;

SALSBURG, DS .

MUTATION RESEARCH, 1980, 72 (03) :447-474

[3] METHODS FOR DETECTING CARCINOGENS AND MUTAGENS WITH SALMONELLA-MAMMALIAN-MICROSOME MUTAGENICITY TEST [J].

AMES, BN ;

MCCANN, J ;

YAMASAKI, E .

MUTATION RESEARCH, 1975, 31 (06) :347-363

[4] A combined subchronic (90-day) toxicity and neurotoxicity study of a single-cell source of docosahexaenoic acid triglyceride (DHASCO® oil) [J].

Arterburn, LM ;

Boswell, KD ;

Koskelo, EK ;

Kassner, SL ;

Kelly, C ;

Kyle, DJ .

FOOD AND CHEMICAL TOXICOLOGY, 2000, 38 (01) :35-49

[5] Visual acuity and the essentiality of docosahexaenoic acid and arachidonic acid in the diet of term infants [J].

Birch, EE ;

Hoffman, DR ;

Uauy, R ;

Birch, DG ;

Prestidge, C .

PEDIATRIC RESEARCH, 1998, 44 (02) :201-209

[6]

BIRCH EE, 2000, IN PRESS J PEDIAT NE

[7] Preclinical evaluation of single-cell oils that are highly enriched with arachidonic acid and docosahexaenoic acid [J].

Boswell, K ;

Koskelo, EK ;

Carl, L ;

Glaza, S ;

Hensen, DJ ;

Williams, KD ;

Kyle, DJ .

FOOD AND CHEMICAL TOXICOLOGY, 1996, 34 (07) :585-593

[8] Evaluation of single-cell sources of docosahexaenoic acid and arachidonic acid:: 3-month rat oral safety study with an in utero phase [J].

Burns, RA ;

Wibert, GJ ;

Diersen-Schade, DA ;

Kelly, CM .

FOOD AND CHEMICAL TOXICOLOGY, 1999, 37 (01) :23-36

[9] VISUAL-ACUITY DEVELOPMENT IN HEALTHY PRETERM INFANTS - EFFECT OF MARINE-OIL SUPPLEMENTATION [J].

CARLSON, SE ;

WERKMAN, SH ;

RHODES, PG ;

TOLLEY, EA .

AMERICAN JOURNAL OF CLINICAL NUTRITION, 1993, 58 (01) :35-42

[10] GUIDE FOR PERFORMING THE MOUSE LYMPHOMA ASSAY FOR MAMMALIAN-CELL MUTAGENICITY [J].

CLIVE, D ;

CASPARY, W ;

KIRBY, PE ;

KREHL, R ;

MOORE, M ;

MAYO, J ;

OBERLY, TJ .

MUTATION RESEARCH, 1987, 189 (02) :143-156