Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans

被引：215

作者：

Yang, L ^{[1
]}

Ukil, L ^{[1
]}

Osmani, A ^{[1
]}

Nahm, F ^{[1
]}

Davies, J ^{[1
]}

De Souza, CPC ^{[1
]}

Dou, XW ^{[1
]}

Perez-Balaguer, A ^{[1
]}

Osmani, SA ^{[1
]}

机构：

[1] Ohio State Univ, Dept Mol Genet, Columbus, OH 43210 USA

来源：

EUKARYOTIC CELL | 2004年 / 3卷 / 05期

关键词：

D O I：

10.1128/EC.3.5.1359-1362.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A method to rapidly generate gene replacement constructs by fusion PCR is described for Aspergillus nidulans. The utility of the approach is demonstrated by green fluorescent protein (GFP) tagging of A. nidulans ndc80 to visualize centromeres through the cell cycle. The methodology makes possible large-scale GFP tagging, promoter swapping, and deletion analysis of A. nidulans.

引用

页码：1359 / 1362

页数：4

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