The indeterminate gametophyte1 gene of maize encodes a LOB domain protein required for embryo sac and leaf development

被引:191
作者
Evans, Matthew M. S. [1 ]
机构
[1] Carnegie Inst Washington, Dept Plant Biol, Stanford, CA 94305 USA
关键词
D O I
10.1105/tpc.106.047506
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Angiosperm embryo sac development begins with a phase of free nuclear division followed by cellularization and differentiation of cell types. The indeterminate gametophyte1 (ig1) gene of maize (Zea mays) restricts the proliferative phase of female gametophyte development. ig1 mutant female gametophytes have a prolonged phase of free nuclear divisions leading to a variety of embryo sac abnormalities, including extra egg cells, extra polar nuclei, and extra synergids. Positional cloning of ig1 was performed based on the genome sequence of the orthologous region in rice. ig1 encodes a LATERAL ORGAN BOUNDARIES domain protein with high similarity to ASYMMETRIC LEAVES2 of Arabidopsis thaliana. A second mutant allele of ig1 was identified in a noncomplementation screen using active Mutator transposable element lines. Homozygous ig1 mutants have abnormal leaf morphology as well as abnormal embryo sac development. Affected leaves have disrupted abaxial-adaxial polarity and fail to repress the expression of meristem-specific knotted-like homeobox (knox) genes in leaf primordia, causing a proliferative, stem cell identity to persist in these cells. Despite the superficial similarity of ig1-O leaves and embryo sacs, ectopic knox gene expression cannot be detected in ig1-O embryo sacs.
引用
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页码:46 / 62
页数:17
相关论文
共 58 条
[1]   A heterodimerizing leucine zipper coiled coil system for examining the specificity of a position interactions: Amino acids I, V, L, N, A, and K [J].
Acharya, A ;
Ruvinov, SB ;
Gal, J ;
Moll, JR ;
Vinson, C .
BIOCHEMISTRY, 2002, 41 (48) :14122-14131
[2]  
[Anonymous], NEURAL INFORM PROCES
[3]   Gametophyte development in ferns [J].
Banks, JA .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1999, 50 :163-+
[4]   ramosa2 encodes a LATERAL ORGAN BOUNDARY domain protein that determines the fate of stem cells in branch meristems of maize [J].
Bortiri, E ;
Chuck, G ;
Vollbrecht, E ;
Rocheford, T ;
Martienssen, R ;
Hake, S .
PLANT CELL, 2006, 18 (03) :574-585
[5]   Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis [J].
Byrne, ME ;
Barley, R ;
Curtis, M ;
Arroyo, JM ;
Dunham, M ;
Hudson, A ;
Martienssen, RA .
NATURE, 2000, 408 (6815) :967-971
[6]  
COVE DJ, 1993, PLANT CELL, V5, P1483
[7]   Use of the transposon Ac as a gene-searching engine in the maize genome [J].
Cowperthwaite, M ;
Park, W ;
Xu, ZN ;
Yan, XH ;
Maurais, SC ;
Dooner, HK .
PLANT CELL, 2002, 14 (03) :713-726
[8]   Dimerization specificity of all 67 B-ZIP motifs in Arabidopsis thaliana:: a comparison to Homo sapiens B-ZIP motifs [J].
Deppmann, CD ;
Acharya, A ;
Rishi, V ;
Wobbes, B ;
Smeekens, S ;
Taparowsky, EJ ;
Vinson, C .
NUCLEIC ACIDS RESEARCH, 2004, 32 (11) :3435-3445
[9]   Development and function of the angiosperm female gametophyte [J].
Drews, GN ;
Yadegari, R .
ANNUAL REVIEW OF GENETICS, 2002, 36 :99-124
[10]   Plant retinoblastoma homologues control nuclear proliferation in the female gametophyte [J].
Ebel, C ;
Mariconti, L ;
Gruissem, W .
NATURE, 2004, 429 (6993) :776-780