Confocal time-resolved fluorescence anisotropy imaging

被引：2

作者：

Bader, Arjen N. ^{[1
]}

Hofman, Erik G. ^{[2
]}

Henegouwen, Paul van Bergen en ^{[2
]}

Gerritsen, Hans C. ^{[1
]}

机构：

[1] Univ Utrecht, Mol Biophys, Postbus 80000, NL-3508 TA Utrecht, Netherlands

[2] Univ Utrecht, Cellular Architecture & Dynam, NL-3508 TA Utrecht, Netherlands

来源：

IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES V | 2007年 / 6441卷

关键词：

D O I：

10.1117/12.702031

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A confocal time-resolved fluorescence anisotropy imaging set-up is presented. It combines a confocal laser scanning microscope equipped with a pulsed laser and two time gated detection systems with 4 gates each (LiMo, originally developed for FLIM). The anisotropy decays obtained with the time gating system yield results that compare well with the high time-resolution (non-imaging) decays recorded using Time Correlated Single Photon Counting. Time resolved anisotropy imaging experiments on cells expressing GPI-GFP were carried out. Clear distinction could be made between the anisotropy in the plasma membrane and in the interior of the cell.

引用

页数：9

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