Purification and characterization of two voltage-dependent anion channel isoforms from plant seeds

被引：28

作者：

Abrecht, H

Wattiez, R

Ruysschaert, JM

Homblé, F ^{[1
]}

机构：

[1] Free Univ Brussels, Fac Sci, Lab Physiol Vegetale, B-1050 Brussels, Belgium

[2] Free Univ Brussels, Fac Sci, Chim Phys Macromol Interfaces Lab, B-1050 Brussels, Belgium

[3] Univ Mons, Fac Sci, Biochim Lab, B-7000 Mons, Belgium

来源：

PLANT PHYSIOLOGY | 2000年 / 124卷 / 03期

关键词：

D O I：

10.1104/pp.124.3.1181

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Mitochondria were isolated from imbibed seeds of lentil (Lens culinaris) and Phaseolus vulgaris. We copurified two voltage-dependent anion channel from detergent solubilized mitochondria in a single purification step using hydroxyapatite. The two isoforms from P. vulgaris were separated by chromatofocusing chromatography in 4 M urea without any loss of channel activity, Channel activity of each isoform was characterized upon reconstitution into diphytanoyl phosphatidylcholine planar lipid bilayers. Both isoforms form large conductance channels that are slightly anion selective and display cation selective substates.

引用

页码：1181 / 1190

页数：10

共 44 条

[1] PORIN INTERACTION WITH HEXOKINASE AND GLYCEROL KINASE - METABOLIC MICROCOMPARTMENTATION AT THE OUTER MITOCHONDRIAL-MEMBRANE [J].