Isolation and molecular characterization of the rat MR1 homologue, a non-MHC-linked class I-related gene

被引：26

作者：

Walter, L ^{[1
]}

Günther, E ^{[1
]}

机构：

[1] Univ Gottingen, Immungenet Abt, D-37073 Gottingen, Germany

来源：

IMMUNOGENETICS | 1998年 / 47卷 / 06期

关键词：

rat; major histocompatibility complex class I; Mrl; evolution;

D O I：

10.1007/s002510050385

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We isolated and analyzed a new rat gene which is homologous to the recently described human major histocompatibility complex class I-related gene MR1. The deduced amino acid sequence of the rat Mr1 gene shows conserved cysteine residues typical of class I genes as well as conserved beta(2)-microglobulin and CD8 contact sites. Analysis of partial DNA sequences and restriction fragment length polymorphism patterns of several inbred rat strains indicate that Mr1 is not polymorphic. Mr1 is a single-copy gene, which could be mapped to rat chromosome 13 by cosegregation analysis of Mr1 and a microsatellite marker in the renin (Ren) gene in double-backcross hybrids. The recombination frequency between both genes was determined to be 14.7% (4.9-31.1, 95% confidence limits). Expression analysis revealed various Mr1 transcripts in each organ tested and occurrence of alternative splicing.

引用

页码：477 / 482

页数：6

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