Geographical segregation of the neurotoxin-producing cyanobacterium Anabaena circinalis

被引:83
作者
Beltran, EC [1 ]
Neilan, BA [1 ]
机构
[1] Univ New S Wales, Sch Microbiol & Immunol, Sydney, NSW 2052, Australia
关键词
D O I
10.1128/AEM.66.10.4468-4474.2000
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Blooms of the cyanobacterium Anabaena circinalis are a major worldwide problem due to their production of a range of toxins, in particular the neurotoxins anatoxin-a and paralytic shellfish poisons (PSPs). Although there is a worldwide distribution of A. circinalis, there is a geographical segregation of neurotoxin production. American and European isolates of A. circinalis produce only anatoxin-a, while Australian isolates exclusively produce PSPs, The reason for this geographical segregation of neurotoxin production by A. circinalis is unknown. The phylogenetic structure of A. circinalis was determined by analyzing 16S rRNA gene sequences. A. circinalis was found to form a monophyletic group of international distribution. However, the PSP- and non-PSP-producing A. circinalis formed two distinct 16S rRNA gene clusters. A molecular probe was designed, allowing the identification of A. circinalis from cultured and uncultured environmental samples. In addition, probes targeting the predominantly PSP-producing or non-PSP-producing clusters were designed for the characterization of A. circinalis isolates as potential PSP producers.
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页码:4468 / 4474
页数:7
相关论文
共 41 条
[1]   RED TIDES [J].
ANDERSON, DM .
SCIENTIFIC AMERICAN, 1994, 271 (02) :62-68
[2]  
BAKER PD, 1994, AUST J MAR FRESH RES, V45, P773
[3]  
BAKER PD, 1993, 893 AUSTR CTR WAT QU
[4]  
BOWLING L, 1994, AUST J MAR FRESH RES, V45, P737
[5]   CYANOBACTERIA SECONDARY METABOLITES - THE CYANOTOXINS [J].
CARMICHAEL, WW .
JOURNAL OF APPLIED BACTERIOLOGY, 1992, 72 (06) :445-459
[6]   PHARMACOLOGY OF ANATOXIN-A, PRODUCED BY THE FRESHWATER CYANOPHYTE ANABAENA-FLOS-AQUAE NRC-44-1 [J].
CARMICHAEL, WW ;
BIGGS, DF ;
PETERSON, MA .
TOXICON, 1979, 17 (03) :229-236
[7]   TOXINS OF CYANOBACTERIA [J].
CARMICHAEL, WW .
SCIENTIFIC AMERICAN, 1994, 270 (01) :78-86
[8]  
FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x
[9]   Evidence for production of paralytic shellfish toxins by bacteria associated with Alexandrium spp (Dinophyta) in culture [J].
Gallacher, S ;
Flynn, KJ ;
Franco, JM ;
Brueggemann, EE ;
Hines, HB .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (01) :239-245
[10]  
Gibson C., 1982, BIOL CYANOBACTERIA, P463