Separation of basic solutes by reversed-phase capillary electrochromatography

被引：78

作者：

Dittmann, MM ^{[1
]}

Masuch, K ^{[1
]}

Rozing, GP ^{[1
]}

机构：

[1] Hewlett Packard GMBH, Waldbronn Analyt Div, D-76337 Waldbronn, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 887卷 / 1-2期

关键词：

electrochromatography; retention models; basic solutes;

D O I：

10.1016/S0021-9673(00)00325-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The separation of basic solutes at low pH by capillary electrochromatography (CEC) has been investigated. The feasibility of separation of basic solutes by CEC was demonstrated. Influence of operational parameters, solvent composition, pH, temperature on retention and selectivity of the separation of a mixture of basic, neutral and acidic drug standards has been investigated. The observed elution behavior has been modeled to account for both chromatographic retention and differential electrophoretic mobility of the solutes. This model was verified experimentally. It is demonstrated in this work that the elution window of solutes in reversed-phase CEC is expanded to range from -1 to infinity. (C) 2000 Published by Elsevier Science B.V.

引用

页码：209 / 221

页数：13

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