Genetic evidence for highly diversified bacterial populations in wastewater sludge during biological leaching of metals

被引:4
作者
Fournier, D
Lemieux, R
Couillard, D
机构
[1] INRA, St Foy, PQ G1V 4C7, Canada
[2] Canadian Red Cross Soc, Quebec Transfus Ctr, St Foy, PQ G1V 4M3, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
D O I
10.1023/A:1005370828275
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
By coupling the sensitivity of 16S rRNA gene amplification by PCR (< 30 bacteria/ml), and the resolution of denaturing gradient gel electrophoresis (DGGE), we studied the bacterial populations involved in the metal biological leaching of wastewater sludge. We were thus able to enumerate more than 20 different bands, revealing a bacterial diversity undetected by cultivation techniques.
引用
收藏
页码:27 / 31
页数:5
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