Design of peptide that recognizes double-stranded DNA

被引：4

作者：

Fukumori, T

Morita, Y

Tamiya, E

Yokoyama, K

机构：

[1] Japan Adv Inst Sci & Technol, Sch Mat Sci, Tatsunokuchi, Ishikawa 9231292, Japan

[2] Natl Inst Adv Ind Sci & Technol, Lab Adv Bioelect, Tsukuba, Ibaraki 3058562, Japan

来源：

ANALYTICAL SCIENCES | 2003年 / 19卷 / 01期

关键词：

D O I：

10.2116/analsci.19.181

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel molecular tool for double-stranded (ds) DNA detection using synthetic peptide is described. The peptide was designed based on the DNA binding domain of the lambda phage CRO repressor (CRO). The designed peptides contain helix-turn-helix (HTH), which is DNA binding motif. A cyclic peptide and a mutant peptide based on CRO were also designed, and the resulting affinity for dsDNA was increased. Furthermore, native amino acids of the peptide were replaced with arginine to increase the affinity for dsDNA. The affinity of these peptides for DNA binding was assessed by surface plasmon resonance (SPR) technique.

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页码：181 / 183

页数：3

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