Molecular cloning and biological activity of α-, β-, and γ-megaspermin, three elicitins secreted by Phytophthora megasperma H2O

被引:47
作者
Baillieul, F
de Ruffray, P
Kauffmann, S
机构
[1] Univ Strasbourg, CNRS, Inst Biol Mol Plantes, F-67084 Strasbourg, France
[2] Univ Reims, Unite Format & Rech Sci, Lab Biol & Physiol Vegetales, F-51687 Reims, France
关键词
D O I
10.1104/pp.012658
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We report on the molecular cloning of the Phytophthora megasperma H20 (PmH20) glycoprotein shown previously as an inducer of the hypersensitive response, of localized acquired resistance and of systemic acquired resistance in tobacco (Nicotiana tabacum), and of the PmH20 alpha- and beta-megaspermin, two elicitins of class I-A and I-B, respectively. The structure of the glycoprotein shows a signal peptide of 20 amino acids followed by the typical elicitin 98-amino acid-long domain and a 77-amino acid-long C-terminal domain carrying an O-glycosylated moiety. The molecular mass deduced from the translated cDNA sequence is 14,920 and 18,676 D as determined by mass spectrometry. This structure together with multiple sequence alignments and phylogenetic analyses indicate that the glycoprotein belongs to class III elicitins. It is the first class III elicitin protein characterized, which we named gamma-megaspermin. We compared the biological activity of the three PmH20 elicitins when applied to tobacco cv Samsun NN plants. Although alpha- and gamma-megaspermin were similarly active, beta-megaspermin was the most active in inducing the hypersensitive response and localized acquired resistance, which was assessed by measuring the levels of acidic and basic pathogenesis-related proteins and of the antioxidant phytoalexin scopoletin. The three elicitins induced similar levels of systemic acquired resistance measured as the expression of acidic PR proteins and is increased resistance to challenge tobacco mosaic virus infection.
引用
收藏
页码:155 / 166
页数:12
相关论文
共 50 条
[1]   A NEW ELICITOR OF THE HYPERSENSITIVE RESPONSE IN TOBACCO - A FUNGAL GLYCOPROTEIN ELICITS CELL-DEATH, EXPRESSION OF DEFENSE GENES, PRODUCTION OF SALICYLIC-ACID, AND INDUCTION OF SYSTEMIC ACQUIRED-RESISTANCE [J].
BAILLIEUL, F ;
GENETET, I ;
KOPP, M ;
SAINDRENAN, P ;
FRITIG, B ;
KAUFFMANN, S .
PLANT JOURNAL, 1995, 8 (04) :551-560
[2]   Occurrence among Phytophthora species of a glycoprotein eliciting a hypersensitive response in tobacco and its relationships with elicitins [J].
Baillieul, F ;
Fritig, B ;
Kauffmann, S .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1996, 9 (03) :214-216
[3]  
BAILLIEUL F, 1994, CURR PLANT SCI BIOT, V21, P323
[4]   Disruption of microtubular cytoskeleton induced by cryptogein, an elicitor of hypersensitive response in tobacco cells [J].
Binet, MN ;
Humbert, C ;
Lecourieux, D ;
Vantard, M ;
Pugin, A .
PLANT PHYSIOLOGY, 2001, 125 (02) :564-572
[5]   RESPONSES OF CULTURED TOBACCO CELLS TO CRYPTOGEIN, A PROTEINACEOUS ELICITOR FROM PHYTOPHTHORA-CRYPTOGEA - POSSIBLE PLASMALEMMA INVOLVEMENT [J].
BLEIN, JP ;
MILAT, ML ;
RICCI, P .
PLANT PHYSIOLOGY, 1991, 95 (02) :486-491
[6]   Acquired resistance triggered by elicitins in tobacco and other plants [J].
Bonnet, P ;
Bourdon, E ;
Ponchet, M ;
Blein, JP ;
Ricci, P .
EUROPEAN JOURNAL OF PLANT PATHOLOGY, 1996, 102 (02) :181-192
[7]   Comparison of binding properties and early biological effects of elicitins in tobacco cells [J].
Bourque, S ;
Ponchet, M ;
Binet, MN ;
Ricci, P ;
Pugin, A ;
Lebrun-Garcia, A .
PLANT PHYSIOLOGY, 1998, 118 (04) :1317-1326
[8]   Biological and molecular comparison between localized and systemic acquired resistance induced in tobacco by a Phytophthora megasperma glycoprotein elicitin [J].
Cordelier, S ;
de Ruffray, P ;
Fritig, B ;
Kauffmann, S .
PLANT MOLECULAR BIOLOGY, 2003, 51 (01) :109-118
[9]   Relationship between localized acquired resistance (LAR) and the hypersensitive response (HR): HR is necessary for LAR to occur and salicylic acid is not sufficient to trigger LAR [J].
Costet, L ;
Cordelier, S ;
Dorey, S ;
Baillieul, F ;
Fritig, B ;
Kauffmann, S .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1999, 12 (08) :655-662
[10]   Scopoletin expression in elicitor-treated and tobacco mosaic virus-infected tobacco plants [J].
Costet, L ;
Fritig, B ;
Kauffmann, S .
PHYSIOLOGIA PLANTARUM, 2002, 115 (02) :228-235