Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by Burkholderia sacchari using wheat straw hydrolysates and gamma-butyrolactone

被引:47
作者
Cesario, M. Teresa [1 ]
Raposo, Rodrigo S. [1 ]
de Almeida, M. Catarina M. D. [1 ,3 ]
van Keulen, Frederik [2 ]
Ferreira, Bruno S. [2 ]
Telo, Joao P. [4 ]
da Fonseca, M. Manuela R. [1 ]
机构
[1] Univ Lisbon, Inst Super Tecn, Inst Biotechnol & Bioengn, P-1049001 Lisbon, Portugal
[2] Biotrend Inovacao & Engn Biotecnol SA, P-3060197 Cantanhede, Portugal
[3] ISCSEM, Ctr Invest Interdisciplinar Egas Moniz, P-2829511 Monte De Caparica, Portugal
[4] Univ Lisbon, Inst Super Tecn, Ctr Quim Estrutural, P-1049001 Lisbon, Portugal
基金
欧盟第七框架计划;
关键词
Burkholderia sacchari; Poly(3-hydroxybutyrate-co-4-hydroxybutyrate); Wheat straw lignocellulosic hydrolysates; ALCALIGENES-EUTROPHUS; RALSTONIA-EUTROPHA; MICROBIAL SYNTHESIS; BIOSYNTHESIS; POLYHYDROXYALKANOATES; 4-HYDROXYBUTYRATE; GLUCOSE; POLY-3-HYDROXYBUTYRATE; ACIDOVORANS; COPOLYMER;
D O I
10.1016/j.ijbiomac.2014.04.054
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Burkholderia sacchari DSM 17165 is able to grow and produce poly(3-hydroxybutyrate) both on hexoses and pentoses. In a previous study, wheat straw lignocellulosic hydrolysates (WSH) containing high C6 and C5 sugar concentrations were shown to be excellent carbon sources for P(3HB) production. Using a similar feeding strategy developed for P(3HB) production based on WSH, fed-batch cultures were developed aiming at the production of the copolymer P(3HB-co-4HB) (poly(3-hydroxybutyrate-co-4-hydroxybutyrate)) by B. sacchari. The ability of this strain to synthesize P(3HB-co-4HB) was first shown in shake flasks using gamma-butyrolactone (GBL) as precursor of the 4HB units. Fed-batch cultures using glucose as carbon source (control) and GBL were developed to achieve high copolymer productivities and 4HB incorporations. The attained P(3HB-co-4HB) productivity and 4HB molar% were 0.7 g/(L h) and 4.7 molar%, respectively. The 4HB incorporation was improved to 6.3 and 11.8 molar% by addition of 2 g/L propionic and acetic acid, respectively. When WSH were used as carbon source under the same feeding conditions, the values achieved were 0.5 g/(L h) and 5.0 molar%, respectively. Burkholderia sacchari, a strain able to produce biopolymers based on xylose-rich lignocellulosic hydrolysates, is for the first time reported to produce P(3HB-co-4HB) using gamma butyrolactone as precursor. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:59 / 67
页数:9
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