Shorter T-DNA or additional virulence genes improve Agrobacterium-mediated transformation

被引：26

作者：

Park, SH ^{[1
]}

Lee, BM ^{[1
]}

Salas, MG ^{[1
]}

Srivatanakul, M ^{[1
]}

Smith, RH ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA

来源：

THEORETICAL AND APPLIED GENETICS | 2000年 / 101卷 / 07期

关键词：

Agrobacterium; rice; cotton; tobacco; virulence genes;

D O I：

10.1007/s001220051575

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The effect of additional virulence (vii) genes and size of plasmid T-DNA in Agrobacterium tumefaciens was investigated for their impact on transformation efficiency. Transformation efficiency in tobacco, cotton, and rice was increased when the T-DNA was 4.3 kb compared to 8.4 kb in size. However, when additional vir-G, virGN54D, virE, or virE/virG plasmids were included with the 8.4-kb T-DNA, transformation frequencies in all cases were increased over that of the shorter T-DNA without additional vir plasmids. The use of virE, virG or virGN54D copies enhanced transformation efficiency; however, the most significant increase of transformation efficiency in all three plant species was observed when the virE/virG plasmid was used for infection. The virE/vir-G plasmid dramatically enhanced the efficiency of Agrobacterium-mediated gene transfer; moreover, this plasmid appears to have broad efficiency since it was consistently effective on two different dicotyledon species as well as a monocotyledon species.

引用

页码：1015 / 1020

页数：6

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