Study on the structure and properties of wool keratin regenerated from formic acid

被引：244

作者：

Aluigi, A.

Zoccola, M.

Vineis, C.

Tonin, C.

Ferrero, F.

Canetti, M.

机构：

[1] CNR ISMAC, Inst Macromol Studies, I-13900 Biella, Italy

[2] CNR ISMAC, Inst Macromol Studies, I-20133 Milan, Italy

[3] Politecn Torino, Dept Mat Sci & Chem Engn, I-10129 Turin, Italy

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2007年 / 41卷 / 03期

关键词：

wool keratin; formic acid; solution stability; protein structure;

D O I：

10.1016/j.ijbiomac.2007.03.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Structural characteristics of keratin regenerated from water (KW) and from formic (KF) acid solutions were compared. Amino acid composition and molecular weight distribution of KW and KF samples were studied by high performance liquid chromatography (HPLC) and SDS-PAGE electrophoresis. Turbidity measurement showed that keratin dissolved in formic acid forms transparent and stable solutions and no flocculation occurs. In addition, because of its good solvation properties, studied by viscosity measurements, formic acid can be used as a co-solvent to prepare keratin-based blend solutions. Structural studies carried out by X-ray diffraction (XRD), Fourier transform infrared (FT-IR) and near infrared (NIR) suggest that formic acid stabilizes the beta-sheet structure. Thermogravimetric analysis (TGA) reveals a higher thermal stability of keratin regenerated from formic acid with respect to keratin regenerated from water. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：266 / 273

页数：8

共 34 条

[1] Flow behavior of regenerated wool-keratin proteins in different mediums [J].