Gene expression of gonadotrophin-releasing hormone, but not its receptor, in human endometrium and decidua

被引：23

作者：

Ikeda, M ^{[1
]}

Taga, M ^{[1
]}

Kurogi, K ^{[1
]}

Minaguchi, H ^{[1
]}

机构：

[1] Yokohama City Univ, Sch Med, Dept Obstet & Gynecol, Yokohama, Kanagawa 236, Japan

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1997年 / 135卷 / 02期

关键词：

gene expression; gonadotrophin-releasing hormone; gonadotrophin-releasing hormone receptor; uterus; pregnancy;

D O I：

10.1016/S0303-7207(97)00202-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Gonadotropin-releasing hormone (GnRH) has been reported to exist in extrahypothalamic tissues such as the placenta, gonads and mammary glands. While we have reported the presence of GnRH-mRNA in the rodent uterus, there have been no reports concerning gene expression of GnRH and its receptor (GnRH-R) in human endometrial tissue. In order to investigate the role of GnRH as a local regulator in the human endometrium, we examined the gene for GnRH and GnRH-R in non-pregnant endometrium and decidua of early pregnancy. Using reverse transcriptase-polymerase chain reaction (RT-PCR) and Southern blot analysis we found GnRH-mRNA but not GnRH-R-mRNA transcripts in the human endometrium and decidua at 7-9 weeks gestation. This is the first report that suggests GnRH gene expression in the human endometrium/decidua. (C) 1997 Elsevier Science Ireland Ltd.

引用

页码：165 / 168

页数：4

共 22 条

[1]

AMARANT T, 1982, EUR J BIOCHEM, V127, P647, DOI 10.1111/j.1432-1033.1982.tb06921.x

[2] OVARIAN GONADOTROPIN-RELEASING HORMONE-LIKE PROTEIN(S) - DEMONSTRATION AND CHARACTERIZATION [J].

ATEN, RF ;

WILLIAMS, AT ;

BEHRMAN, HR ;

WOLIN, DL .

ENDOCRINOLOGY, 1986, 118 (03) :961-967

[3]

Chatzaki E, 1996, CANCER RES, V56, P2059

[4] Gonadotropin-releasing hormone (GnRH) and GnRH receptor gene expression in human myometrium and leiomyomata and the direct action of GnRH analogs on myometrial smooth muscle cells and interaction with ovarian steroids in vitro [J].

Chegini, N ;

Rong, H ;

Dou, QC ;

Kipersztok, S ;

Williams, RS .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1996, 81 (09) :3215-3221

[5] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[6] RAT GRANULOSA-CELLS EXPRESS THE GONADOTROPIN-RELEASING-HORMONE GENE - EVIDENCE FROM INSITU HYBRIDIZATION HISTOCHEMISTRY [J].

CLAYTON, RN ;

ECCLESTON, L ;

GOSSARD, F ;

THALBARD, JC ;

MOREL, G .

JOURNAL OF MOLECULAR ENDOCRINOLOGY, 1992, 9 (03) :189-195

[7] GONADOTROPIN-RELEASING HORMONE BINDING-SITES IN HUMAN-BREAST CARCINOMA [J].

EIDNE, KA ;

FLANAGAN, CA ;

MILLAR, RP .

SCIENCE, 1985, 229 (4717) :989-991

[8]

Fraser H M, 1989, LHRH ITS ANALOGUES, P64

[9] EXTRAPITUITARY ACTIONS OF GONADOTROPIN-RELEASING HORMONE [J].

HSUEH, AJW ;

JONES, PBC .

ENDOCRINE REVIEWS, 1981, 2 (04) :437-461

[10] DETECTION OF MESSENGER-RNA FOR GONADOTROPIN-RELEASING-HORMONE (GNRH) BUT NOT FOR GNRH RECEPTORS IN MOUSE MAMMARY-GLANDS [J].

IKEDA, M ;

TAGA, M ;

SAKAKIBARA, H ;

MINAGUCHI, H ;

VONDERHAAR, BK .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 207 (02) :800-806

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