On-line concentration of neutral analytes for micellar electrokinetic chromatography - II. Reversed electrode polarity stacking mode

被引：82

作者：

Quirino, JP ^{[1
]}

Terabe, S ^{[1
]}

机构：

[1] Himeji Inst Technol, Fac Sci, Kamigoori, Hyogo 67812, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 791卷 / 1-2期

关键词：

sample stacking; sample handling; injection methods;

D O I：

10.1016/S0021-9673(97)00796-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Basic conditions for the on-line neutral sample concentration by reversed electrode polarity stacking mode for micellar electrokinetic chromatography are developed theoretically and experimentally verified. Stacking is mainly dependent on analyte retention factors and nature of the pseudostationary phases. More than an order of magnitude improvement in concentration detection limit is achieved for resorcinol using high molecular mass surfactants. (C) 1997 Elsevier Science B.V.

引用

页码：255 / 267

页数：13

共 14 条

[1] LARGE-VOLUME STACKING OF ANIONS IN CAPILLARY ELECTROPHORESIS USING AN ELECTROOSMOTIC FLOW MODIFIER AS A PUMP [J].

BURGI, DS .

ANALYTICAL CHEMISTRY, 1993, 65 (24) :3726-3729

[2] APPLICATION OF SAMPLE STACKING TO GRAVITY INJECTION IN CAPILLARY ELECTROPHORESIS [J].

BURGI, DS ;

CHIEN, RL .

JOURNAL OF MICROCOLUMN SEPARATIONS, 1991, 3 (03) :199-202

[3] OPTIMIZATION IN SAMPLE STACKING FOR HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS [J].

BURGI, DS ;

CHIEN, RL .

ANALYTICAL CHEMISTRY, 1991, 63 (18) :2042-2047

[4] FIELD AMPLIFIED SAMPLE INJECTION IN HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS [J].

CHIEN, RL ;

BURGI, DS .

JOURNAL OF CHROMATOGRAPHY, 1991, 559 (1-2) :141-152

[5] ELECTROOSMOTIC PROPERTIES AND PEAK BROADENING IN FIELD-AMPLIFIED CAPILLARY ELECTROPHORESIS [J].

CHIEN, RL ;

HELMER, JC .

ANALYTICAL CHEMISTRY, 1991, 63 (14) :1354-1361

[6] SAMPLE STACKING OF AN EXTREMELY LARGE INJECTION VOLUME IN HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS [J].

CHIEN, RL ;

BURGI, DS .

ANALYTICAL CHEMISTRY, 1992, 64 (09) :1046-1050

[7] FIELD-AMPLIFIED POLARITY-SWITCHING SAMPLE INJECTION IN HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS [J].

CHIEN, RL ;

BURGI, DS .

JOURNAL OF CHROMATOGRAPHY, 1991, 559 (1-2) :153-161

[8] ON-COLUMN SAMPLE CONCENTRATION USING FIELD AMPLIFICATION IN CZE [J].

CHIEN, RL ;

BURGI, DS .

ANALYTICAL CHEMISTRY, 1992, 64 (08) :A489-A496

[9] FIELD-AMPLIFIED SAMPLE STACKING IN MICELLAR ELECTROKINETIC CHROMATOGRAPHY FOR ON-COLUMN SAMPLE CONCENTRATION OF NEUTRAL MOLECULES [J].

LIU, ZY ;

SAM, P ;

SIRIMANNE, SR ;

MCCLURE, PC ;

GRAINGER, J ;

PATTERSON, DG .

JOURNAL OF CHROMATOGRAPHY A, 1994, 673 (01) :125-132

[10] ZONE SHARPENING OF NEUTRAL SOLUTES IN MICELLAR ELECTROKINETIC CHROMATOGRAPHY WITH ELECTROKINETIC INJECTION [J].

NIELSEN, KR ;

FOLEY, JP .

JOURNAL OF CHROMATOGRAPHY A, 1994, 686 (02) :283-291

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