An application of carbon nanotubes as immobilization materials for biosensor was described for the first time. Single-wall carbon nanotubes (SWNTs) functionalized with carboxylic acid groups were cast on platinum electrode. The film of SWNTs was used to immobilize glucose oxidase by covalent combine method forming a glucose biosensor. The biosensor showed stable amperometric response at a low applied potential (0.40 V), a wide response range of pH (5.5-8) and good stability (kept 90% activity after 4 months).