Photophysical studies on the interaction of two water-soluble porphyrins with bovine serum albumin. Effects upon the porphyrin triplet state characteristics

被引:69
作者
Borissevitch, IE [1 ]
Tominaga, TT [1 ]
Schmitt, CC [1 ]
机构
[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
water-soluble porphyrins; bovine serum albumin; flash-photolysis; triplet state; binding; aggregates;
D O I
10.1016/S1010-6030(98)00216-0
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The interaction of two metal-free water soluble porphyrins (PPh): meso-tetrakis (p-sulfo-natophenyl)porphyrin (TPPS4) and meso-tetrakis( 4-N-methyl-pyridiniumyl)porphyrin (TMPyP), with bovine serum albumin (BSA) was investigated in the pH range from 4.0 to 8.5 using the flash-photolysis technique in comparison with results obtained by optical absorption, fluorescence and resonance light scattering. It was found that in the presence of BSA, TPPS4 can exist in aqueous solutions as free monomers, aggregates and/or monomers bound to a single BSA molecule and monomers inside the BSA aggregates, while TMPyP does not form aggregates at binding. Binding to BSA transforms the profile of the triplet decay curve from monoexponential to biexponential form with the component lifetimes and relative amplitudes depending on binding characteristics. The triplet lifetime of a bound porphyrin monomer is longer than that of a free one. The aggregation of TPPS4 observed at [TPPS4]/[BSA] > 1 reduces the T-T absorption since the lifetimes of the aggregate excited states are very short and/or quantum yield of the aggregate triplet state is very low. The porphyrin binding to BSA reduces the quenching constants of the porphyrin triplet states by molecular oxygen due to obstacles produced by binding. This effect is especially pronounced for the porphyrin molecules located inside BSA aggregates formed around the porphyrin molecules in excess BSA. (C) 1998 Elsevier Science S.A. All rights reserved.
引用
收藏
页码:201 / 207
页数:7
相关论文
共 23 条
[1]   ATROPISOMER-SPECIFIC FORMATION OF PREMICELLAR PORPHYRIN J-AGGREGATES IN AQUEOUS SURFACTANT SOLUTIONS [J].
BARBER, DC ;
FREITAGBEESTON, RA ;
WHITTEN, DG .
JOURNAL OF PHYSICAL CHEMISTRY, 1991, 95 (10) :4074-4086
[2]   ADVANCES IN PHOTOCHEMICAL APPROACHES FOR BLOOD STERILIZATION [J].
BENHUR, E ;
HOROWITZ, B .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1995, 62 (03) :383-388
[4]  
BORISEVICH JE, 1978, HIGH ENERG CHEM, V5, P474
[5]   Fluorescence and optical absorption study of interaction of two water soluble porphyrins with bovine serum albumin. The role of albumin and porphyrin aggregation [J].
Borissevitch, IE ;
Tominaga, TT ;
Imasato, H ;
Tabak, M .
JOURNAL OF LUMINESCENCE, 1996, 69 (02) :65-76
[6]   Resonance light scattering study of aggregation of two water soluble porphyrins due to their interaction with bovine serum albumin [J].
Borissevitch, IE ;
Tominaga, TT ;
Imasato, H ;
Tabak, M .
ANALYTICA CHIMICA ACTA, 1997, 343 (03) :281-286
[7]   PHARMACEUTICS AND DRUG DELIVERY ASPECTS OF HEME AND PORPHYRIN THERAPY [J].
CANNON, JB .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1993, 82 (05) :435-446
[8]   Excited triplet state photophysics of the sulphonated aluminium phthalocyanines bound to human serum albumin [J].
Foley, MSC ;
Beeby, A ;
Parker, AW ;
Bishop, SM ;
Phillips, D .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1997, 38 (01) :10-17
[9]   DYNAMICS OF THE BINDING OF ACRIDINE-DYES TO DNA INVESTIGATED BY TRIPLET EXCITED-STATE PROBE TECHNIQUES [J].
GEACINTOV, NE ;
WALDMEYER, J ;
KUZMIN, VA ;
KOLUBAYEV, T .
JOURNAL OF PHYSICAL CHEMISTRY, 1981, 85 (24) :3608-3613
[10]   LASER-INDUCED LIGHT ATTENUATION IN SOLUTIONS OF PORPHYRIN AGGREGATES [J].
KHAIRUTDINOV, RF ;
SERPONE, N .
JOURNAL OF PHYSICAL CHEMISTRY, 1995, 99 (31) :11952-11958