Molecular characterization of Coxiella burnetii isolates

被引：54

作者：

Jager, C ^{[1
]}

Willems, H ^{[1
]}

Thiele, D ^{[1
]}

Baljer, G ^{[1
]}

机构：

[1] Univ Giessen, Inst Hyg & Infect Dis Anim, Giessen, Germany

来源：

EPIDEMIOLOGY AND INFECTION | 1998年 / 120卷 / 02期

关键词：

D O I：

10.1017/S0950268897008510

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Restriction fragment length polymorphism (RFLP) was used for the differentiation of 80 Coxiella burnetii isolates derived from animals and humans in Europe, USA, Africa and Asia. After NotI restriction of total C. burnetii DNA and pulsed field gel electrophoresis (PFGE) 20 different restriction patterns were distinguished. The index of discrimination for this typing system was 0.86. Comparison and phylogenetic analysis of the different RFLP patterns revealed evolutionary relationships among groups that corresponded to the geographical origin of the isolates. This finding was confirmed by genetic capping. No correlation between restriction group and virulence of isolates was detected.

引用

页码：157 / 164

页数：8

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