HIV-1 DNA and mRNA concentrations are similar in peripheral blood monocytes and alveolar macrophages in HIV-1-infected individuals

被引:58
作者
Lewin, SR
Kirihara, J
Sonza, S
Irving, L
Mills, J
Crowe, SM
机构
[1] Macfarlane Burnet Ctr Med Res, AIDS Pathogenesis Res Unit, Fairfield, Vic 3078, Australia
[2] Fairfield Infect Dis Hosp, Fairfield, Vic, Australia
[3] Austin Repatriat Med Ctr, Dept Resp Med, Heidelberg, Vic, Australia
[4] Natl Ctr HIV Virol, Fairfield, Vic, Australia
关键词
AIDS; viral load; alveolar macrophages; monocytes; HIV-1; pathogenesis;
D O I
10.1097/00002030-199807000-00008
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Objective: To determine the relative contribution of alveolar macrophages, peripheral blood monocytes (PBM) and peripheral blood lymphocytes (PBL) from HIV-infected individuals to HIV-1 viral load. Methods: Alveolar macrophages were obtained by flexible bronchoscopy, and PBM and PBL by venipuncture from HIV-1-infected individuals. Alveolar macrophages and PBM were purified using immunomagnetic bead selection to deplete CD3+ and CD19+ cells from bronchoalveolar lavage and peripheral blood mononuclear cells, respectively. DNA and mRNA were extracted and gag copy number quantified using polymerase chain reaction (PCR) and reverse transcriptase PCR. The titres of infectious cell-associated HIV-1 in cells were determined by the endpoint dilution coculture technique for alveolar macrophages and PBM. Results: Alveolar macrophages and PBM from HIV-1-infected subjects (n = 11) contained equivalent concentrations of HIV-1 DNA and HIV-1 mRNA as determined by PCR and reverse transcriptase PCR, respectively. Antiretroviral therapy was associated with reduced viral DNA concentrations in alveolar macrophages but not in PBM. PBL had a significantly higher level of proviral DNA and mRNA than alveolar macrophages or PBM. Conclusions: Although alveolar macrophages infected in vitro are more permissive for HIV-1 replication than PBM, this difference could not be demonstrated in vivo. (C) 1998 Lippincott-Raven Publishers.
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页码:719 / 727
页数:9
相关论文
共 37 条
[1]   ENHANCED PRODUCTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 BY IN VITRO-INFECTED ALVEOLAR MACROPHAGES FROM OTHERWISE HEALTHY CIGARETTE SMOKERS [J].
ABBUD, RA ;
FINEGAN, CK ;
GUAY, LA ;
RICH, EA .
JOURNAL OF INFECTIOUS DISEASES, 1995, 172 (03) :859-863
[2]   QUANTITATIVE-ANALYSIS OF HIV-1 PROVIRAL DNA IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS FROM PATIENTS WITH AIDS OR ARC - DECREASE OF PROVIRAL DNA CONTENT FOLLOWING TREATMENT WITH 2',3'-DIDEOXYINOSINE (DDI) [J].
AOKI, S ;
YARCHOAN, R ;
THOMAS, RV ;
PLUDA, JM ;
MARCZYK, K ;
BRODER, S ;
MITSUYA, H .
AIDS RESEARCH AND HUMAN RETROVIRUSES, 1990, 6 (11) :1331-1339
[3]   DIFFERENTIAL PRODUCTION OF TUMOR NECROSIS FACTOR, MACROPHAGE COLONY STIMULATING FACTOR, AND INTERLEUKIN-1 BY HUMAN ALVEOLAR MACROPHAGES [J].
BECKER, S ;
DEVLIN, RB ;
HASKILL, JS .
JOURNAL OF LEUKOCYTE BIOLOGY, 1989, 45 (04) :353-361
[4]   DETECTION OF HTLV-III RNA IN LUNGS OF PATIENTS WITH AIDS AND PULMONARY INVOLVEMENT [J].
CHAYT, KJ ;
HARPER, ME ;
MARSELLE, LM ;
LEWIN, EB ;
ROSE, RM ;
OLESKE, JM ;
EPSTEIN, LG ;
WONGSTAAL, F ;
GALLO, RC .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1986, 256 (17) :2356-2359
[5]   Quantification of latent tissue reservoirs and total body viral load in HIV-1 Infection [J].
Chun, TW ;
Carruth, L ;
Finzi, D ;
Shen, XF ;
DiGiuseppe, JA ;
Taylor, H ;
Hermankova, M ;
Chadwick, K ;
Margolick, J ;
Quinn, TC ;
Kuo, YH ;
Brookmeyer, R ;
Zeiger, MA ;
BarditchCrovo, P ;
Siliciano, RF .
NATURE, 1997, 387 (6629) :183-188
[6]   THE EPIDEMIOLOGY OF HIV-1 INFECTION OF THE LUNG IN AIDS PATIENTS [J].
CLARKE, JR ;
TAYLOR, IK ;
FLEMING, J ;
NUKUNA, A ;
WILLIAMSON, JD ;
MITCHELL, DM .
AIDS, 1993, 7 (04) :555-560
[7]   DETECTION OF HIV-1 IN HUMAN LUNG MACROPHAGES USING THE POLYMERASE CHAIN-REACTION [J].
CLARKE, JR ;
KRISHNAN, V ;
BENNETT, J ;
MITCHELL, D ;
JEFFRIES, DJ .
AIDS, 1990, 4 (11) :1133-1136
[8]  
CLARKE JR, 1994, CLIN EXP IMMUNOL, V96, P182
[9]   HUMAN IMMUNODEFICIENCY VIRUS RECOVERY FROM BRONCHOALVEOLAR LAVAGE FLUID IN PATIENTS WITH AIDS [J].
DEAN, NC ;
GOLDEN, JA ;
EVANS, LA ;
WARNOCK, ML ;
ADDISON, TE ;
HOPEWELL, PC ;
LEVY, JA .
CHEST, 1988, 93 (06) :1176-1179
[10]   HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 IN SPINAL CORDS OF ACQUIRED IMMUNODEFICIENCY SYNDROME PATIENTS WITH MYELOPATHY - EXPRESSION AND REPLICATION IN MACROPHAGES [J].
EILBOTT, DJ ;
PERESS, N ;
BURGER, H ;
LANEVE, D ;
ORENSTEIN, J ;
GENDELMAN, HE ;
SEIDMAN, R ;
WEISER, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (09) :3337-3341