Phospholipase A2 antagonists inhibit constitutive retrograde membrane traffic to the endoplasmic reticulum

被引:50
作者
de Figueiredo, P
Drecktrah, D
Polizotto, RS
Cole, NB
Lippincott-Schwartz, J
Brown, WJ [1 ]
机构
[1] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA
[2] NICHHD, Cell Biol & Metab Branch, NIH, Bethesda, MD 20892 USA
关键词
Golgi complex; membrane tubules; phospholipase A(2); retrograde transport;
D O I
10.1034/j.1600-0854.2000.010608.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Eukaryotic cells contain a variety of cytoplasmic Ca2+-dependent and Ca2+-independent phospholipase A(2)s (PLA(2)s; EC 2.3.1.2.3). However, the physiological roles for many of these ubiquitously-expressed enzymes is unclear or not known. Recently, pharmacological studies have suggested a role for Ca2+-independent PLA(2) (iPLA(2)) enzymes in governing intracellular membrane trafficking events in general and regulating brefeldin A (BFA)-stimulated membrane tubulation and Golgi-to-endoplasmic reticulum (ER) retrograde membrane trafficking, in particular. Here, we extend these studies to show that membrane-permeant iPLA(2) antagonists potently inhibit the normal, constitutive retrograde membrane trafficking from the trans-Golgi network (TGN), Golgi complex, and the ERGIC-53-positive ER-Golgi-intermediate compartment (ERGIC), which occurs in the absence of BFA. Taken together, these results suggest that iPLA(2) enzymes play a general role in regulating, or directly mediating, multiple mammalian membrane trafficking events.
引用
收藏
页码:504 / 511
页数:8
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