High-performance liquid chromatographic determination of ritonavir in human plasma, cerebrospinal fluid and saliva

被引：57

作者：

Hoetelmans, RMW

van Essenberg, M

Profijt, M

Meenhorst, PL

Mulder, JW

Beijnen, JH

机构：

[1] Slotervaart Hosp, Dept Pharm & Pharmacol, NL-1066 EC Amsterdam, Netherlands

[2] Slotervaart Hosp, Dept Internal Med, NL-1066 EC Amsterdam, Netherlands

来源：

JOURNAL OF CHROMATOGRAPHY B | 1998年 / 705卷 / 01期

关键词：

ritonavir;

D O I：

10.1016/S0378-4347(97)00500-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, ion-pair high-performance liquid chromatographic method has been developed and validated for the quantitative determination of the HIV protease inhibitor ritonavir in human plasma, cerebrospinal fluid and saliva. Sample pretreatment consisted of precipitation of proteins with acetonitrile prior to high-performance liquid chromatography with ultraviolet detection at 239 nm. The method has been validated over the range of 50 ng/ml to 50 mu g/ml with use of 100-mu l volumes of sample. The currently described assay has been used successfully for the analysis of ritonavir in plasma, cerebrospinal fluid and saliva in HIV-1 infected patients. (C) 1998 Elsevier Science B.V.

引用

页码：119 / 126

页数：8

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