Population structure of Pseudomonas aeruginosa

被引:241
作者
Wiehlmann, Lutz
Wagner, Gerd
Cramer, Nina
Siebert, Benny
Gudowius, Peter
Morales, Gracia
Koehler, Thilo
van Delden, Christian
Weinel, Christian
Slickers, Peter
Tuemmler, Burkhard
机构
[1] Hannover Med Sch, Klin Forschergrp, D-30625 Hannover, Germany
[2] CLONDIAG Chip Technol GmbH, D-07749 Jena, Germany
[3] Univ Autonoma Madrid, CSIC, Ctr Nacl Biotecnol, Dept Biotecnol Microbiana, E-28049 Madrid, Spain
[4] Ctr Med Univ Geneva, Dept Med Microbial & Med Mol, CH-1211 Geneva, Switzerland
[5] Hop Univ Geneve, Serv Malad Infect, CH-1211 Geneva 14, Switzerland
关键词
bacterial evolution; chip technology; population genetics;
D O I
10.1073/pnas.0609213104
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The metabolically versatile Gram-negative bacterium Pseudomonas aeruginosa inhabits terrestrial, aquatic, animal-, human-, and plant-host-associated environments and is an important causative agent of nosocomial infections, particularly in intensive-care units. The population genetics of A aeruginosa was investigated by an approach that is generally applicable to the rapid, robust, and informative genotyping of bacteria. DNA, amplified from the bacterial colony by circles of multiplex primer extension, is hybridized onto a microarray to yield an electronically portable binary multimarker genotype that represents the core genome by single nucleotide polymorphisms and the accessory genome by markers of genomic islets and islands. The 240 typed A aeruginosa strains of diverse habitats and geographic origin segregated into two large nonoverlapping clusters and 45 isolated clonal complexes with few or no partners. The majority of strains belonged to few dominant clones widespread in disease and environmental habitats. The most frequent genotype was represented by the sequenced strain PA14. Core and accessory genome were found to be nonrandomly assembled in A aeruginosa. Individual clones preferred a specific repertoire of accessory segments. Even the most promiscuous genomic island, pKLC102, had integrated preferentially into a subset of clones. Moreover, some physically distant loci of the core genome, including oriC, showed nonrandom associations of genotypes, whereas other segments in between were freely recombining. Thus, the P. aeruginosa genome is made up of clone-typical segments in core and accessory genome and of blocks in the core with unrestricted gene flow in the population.
引用
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页码:8101 / 8106
页数:6
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