Characterization of Picomole Amounts of Oligosaccharides from Glycoproteins by 1H NMR Spectroscopy

被引：17

作者：

Fellenberg, Meike ^{[1
]}

Coksezen, Atilla ^{[1
]}

Meyer, Bernd ^{[1
]}

机构：

[1] Univ Hamburg, Dept Chem, D-20146 Hamburg, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 14期

关键词：

glycoproteins; NMR spectroscopy; oligosaccharides; structural reporter groups; water suppression; NMR-SPECTROSCOPY; 3D NMR; SUPPRESSION;

D O I：

10.1002/anie.200906680

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

(Graph Presented) Can you see it? NMR spectroscopy is indispensible for structure analysis. It is believed that its use is limited if less than several nanomoles are available. The characterization of oligosaccharides as components of glycoproteins by NMR spectroscopy utilizing significantly less material is highly desirable. Optimization of the sample preparation, water suppression, and instrument setup has enabled only 15 picomoles of oligosaccharides to be analyzed by NMR spectroscopy. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2630 / 2633

页数：4

共 18 条

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[2]

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