Identification of an Arabidopsis mitochondrial succinate-fumarate translocator

被引:49
作者
Catoni, E
Schwab, R
Hilpert, M
Desimone, M
Schwacke, R
Flügge, UI
Schumacher, K
Frommer, WB
机构
[1] ZMBP, Plant Physiol, D-72076 Tubingen, Germany
[2] Univ Cologne, D-50931 Cologne, Germany
关键词
mitochondrial carrier family; succinate transporter; anaplerotic reaction; gluconeogenesis; Arabidopsis thaliana;
D O I
10.1016/S0014-5793(02)03782-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Complementation of a yeast acr1 mutant carrying a deletion of the succinate/fumarate carrier gene enabled functional identification of a mitochondrial succinate translocator from Arabidopsis thaliana (AtmSFC1). Thus complementation of yeast mutants is applicable also for identification and characterization of organellar transporters. Reverse transcription polymerase chain reaction and promoter-GUS fusion showed expression of AtmSFC1 in 2 day old dark grown seedlings, which declined in cotyledons during further development, consistent with a role in export of fumarate for gluconeogenesis during lipid mobilization at early germination of Arabidopsis seeds. In mature plants, expression was found in developing and germinating pollen, suggesting a role in ethanolic fermentation. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:87 / 92
页数:6
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