Purification and partial characterization of the extracellular laccase from Ophiostoma novo-ulmi

被引：20

作者：

Binz, T ^{[1
]}

Canevascini, G ^{[1
]}

机构：

[1] INST BIOL VEGETALE,CH-1700 FRIBOURG,SWITZERLAND

来源：

CURRENT MICROBIOLOGY | 1997年 / 35卷 / 05期

关键词：

D O I：

10.1007/s002849900254

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Ophiostoma ulmi and O. novo-ulmi, both causative agents of Dutch elm disease, can be differentiated by their potential to produce constitutive extracellular laccase. The enzyme has been purified from the culture filtrate to apparent electrophoretic homogeneity and has been partially characterized. The laccase was glycosylated and found to have a molecular mass of 79 kDa or 70 kDa by SDS-PAGE and gel filtration, respectively. The pI, determined by chromatofocusing, was 5.1. Syringaldazine, guaiacol, and other typical laccase substrates were oxidized. No oxidation of tyrosine was detected. NaN3 (0.01%) completely abolished the activity towards 2,6-dimethoxyphenol.

引用

页码：278 / 281

页数：4

共 20 条

[1] CUCURBITACINS - REPRESSORS OF INDUCTION OF LACCASE FORMATION [J].