Enhanced induction of hepatitis C virus-specific cytotoxic T lymphocytes and protective efficacy in mice by DNA vaccination followed by adenovirus boosting in combination with the interleukin-12 expression plasmid

被引：46

作者：

Matsui, M ^{[1
]}

Moriya, O ^{[1
]}

Akatsuka, T ^{[1
]}

机构：

[1] Saitama Med Sch, Dept Microbiol, Moroyama, Saitama 3500495, Japan

来源：

VACCINE | 2003年 / 21卷 / 15期

关键词：

hepatitis C virus; the prime-boost immunization; interleukin-12;

D O I：

10.1016/S0264-410X(02)00704-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We evaluated the prime-boost immunization consisting of hepatitis C virus (HCV)-core expression plasmid (pCEP4-core) and replication-defective adenovirus expressing HCV-core (Adex1SR3ST) for core-specific CTL induction in mice. Compared to a single booster, double boosters after priming enhance CTL induction. The prime-double boosts immunization involving pCEP4-core priming followed by pCEP4-core and Adex1SR3ST boostings (pC/pC/aC) can induce core-specific CTLs as well as other combinations: pC/aC/aC; aC/pC/pC; aC/aC/aC, whereas pC/pC/pC does not induce CTLs. Furthermore, co-administration of interleukin-12 (IL-12) expression plasmid leads to the highly efficient CTL induction and clearance of HCV-core expressing vaccinia virus challenged. Thus, the prime-double boosts immunization together with IL-12 may be promising for HCV vaccine. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：1629 / 1639

页数：11

共 51 条

[1] Mechanisms of cytokine synergy essential for vaccine protection against viral challenge [J].

Ahlers, JD ;

Belyakov, IM ;

Matsui, S ;

Berzofsky, JA .

INTERNATIONAL IMMUNOLOGY, 2001, 13 (07) :897-908

[2] THE NATURAL-HISTORY OF COMMUNITY-ACQUIRED HEPATITIS-C IN THE UNITED-STATES [J].

ALTER, MJ ;

MARGOLIS, HS ;

KRAWCZYNSKI, K ;

JUDSON, FN ;

MARES, A ;

ALEXANDER, WJ ;

HU, PY ;

MILLER, JK ;

GERBER, MA ;

SAMPLINER, RE ;

MEEKS, EL ;

BEACH, MJ .

NEW ENGLAND JOURNAL OF MEDICINE, 1992, 327 (27) :1899-1905

[3] Control of a mucosal challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine [J].

Amara, RR ;

Villinger, F ;

Altman, JD ;

Lydy, SL ;

O'Neil, SP ;

Staprans, SI ;

Montefiori, DC ;

Xu, Y ;

Herndon, JG ;

Wyatt, LS ;

Candido, MA ;

Kozyr, NL ;

Earl, PL ;

Smith, JM ;

Ma, HL ;

Grimm, BD ;

Hulsey, ML ;

Miller, J ;

McClure, HM ;

McNicholl, JM ;

Moss, B ;

Robinson, HL .

SCIENCE, 2001, 292 (5514) :69-74

[4] Hepatitis C virus structural proteins assemble into viruslike particles in insect cells [J].

Baumert, TF ;

Ito, S ;

Wong, DT ;

Liang, TJ .

JOURNAL OF VIROLOGY, 1998, 72 (05) :3827-3836

[5] The importance of local mucosal HIV-specific CD8+ cytotoxic T lymphocytes for resistance to mucosal viral transmission in mice and enhancement of resistance by local administration of IL-12 [J].

Belyakov, IM ;

Ahlers, JD ;

Brandwein, BY ;

Earl, P ;

Kelsall, BL ;

Moss, B ;

Strober, W ;

Berzofsky, JA .

JOURNAL OF CLINICAL INVESTIGATION, 1998, 102 (12) :2072-2081

[6] Hepatitis C virus non-structural protein 3-specific cellular immune responses following single or combined immunization with DNA or recombinant Semliki Forest virus particles [J].

Brinster, C ;

Chen, M ;

Boucreux, D ;

Paranhos-Baccala, G ;

Liljeström, P ;

Lemmonier, F ;

Inchauspé, G .

JOURNAL OF GENERAL VIROLOGY, 2002, 83 :369-381

[7] VACCINATION OF CHIMPANZEES AGAINST INFECTION BY THE HEPATITIS-C VIRUS [J].

CHOO, QL ;

KUO, G ;

RALSTON, R ;

WEINER, A ;

CHIEN, D ;

VANNEST, G ;

HAN, J ;

BERGER, K ;

THUDIUM, K ;

KUO, C ;

KANSOPON, J ;

MCFARLAND, J ;

TABRIZI, A ;

CHING, K ;

MOSS, B ;

CUMMINS, LB ;

HOUGHTON, M ;

MUCHMORE, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (04) :1294-1298

[8] Analysis of a successful immune response against hepatitis C virus [J].

Cooper, S ;

Erickson, AL ;

Adams, EJ ;

Kansopon, J ;

Weiner, AJ ;

Chien, DY ;

Houghton, M ;

Parham, P ;

Walker, CM .

IMMUNITY, 1999, 10 (04) :439-449

[9]

DELBY MA, 2001, J IMMUNOL, V166, P1690

[10] FORMATION AND INTRACELLULAR-LOCALIZATION OF HEPATITIS-C VIRUS ENVELOPE GLYCOPROTEIN COMPLEXES EXPRESSED BY RECOMBINANT VACCINIA AND SINDBIS VIRUSES [J].

DUBUISSON, J ;

HSU, HH ;

CHEUNG, RC ;

GREENBERG, HB ;

RUSSELL, DG ;

RICE, CM .

JOURNAL OF VIROLOGY, 1994, 68 (10) :6147-6160

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