Kinetics of antibodies in sera, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infections

被引：70

作者：

Vazquez, S. ^{[1
]}

Cabezas, S. ^{[1
]}

Perez, A. B. ^{[1
]}

Pupo, M. ^{[1
]}

Ruiz, D. ^{[1
]}

Calzada, N. ^{[1
]}

Bernardo, L. ^{[1
]}

Castro, O. ^{[1
]}

Gonzalez, D. ^{[1
]}

Serrano, T. ^{[1
]}

Sanchez, A. ^{[1
]}

Guzman, M. G. ^{[1
]}

机构：

[1] WHO, PAHO, Collaborat Ctr Dengue & Vectors, Pedro Kouri Trop Med Inst, Havana, Cuba

来源：

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES | 2007年 / 11卷 / 03期

关键词：

dengue antibodies; serum; saliva; urine; ELISA;

D O I：

10.1016/j.ijid.2006.05.005

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: The kinetics of three serological markers (IgM, IgA, and IgG) in serum, saliva, and urine samples from adult patients with primary or secondary dengue infection were studied. Design: Serum, saliva, and urine samples were collected from 22 patients with clinical and confirmed dengue 3 virus infection during the outbreak in Havana City in 2001. They were tested by capture IgM (MAC-ELISA), IgA (AAC-ELISA), and IgE (EAC-ELISA) and IgG ELISA inhibition method (EIM) to detect specific dengue antibodies. Results: Similar kinetics were observed in IgM, IgA, and IgG antibodies in saliva and IgA and IgG in urine samples from secondary cases compared with kinetics in serum samples, although the values were tower. No IgG antibody was detected in saliva and urine samples in primary cases and IgM antibody was not detected in urine samples from either primary or secondary infection. AR secondary cases were positive for IgG in saliva and urine samples at day 7. The kinetics of specific IgE antibodies in primary and secondary cases were different. Conclusions: The kinetics of three serological markers (IgM, IgA, and IgG) in serum, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infection were studied for the first time, showing its behavior and usefulness in dengue virus diagnosis. The specific IgE could play a rote as a serological marker in secondary infections. (C) 2006 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

引用

页码：256 / 262

页数：7

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