Super-activated interferon-regulatory factors can enhance plasmid immunization

被引：31

作者：

Bramson, JL

Dayball, K

Hall, JR

Millar, JB

Miller, M

Wan, YH

Lin, RT

Hiscott, J

机构：

[1] McMaster Univ, Ctr Gene Therapeut, Dept Pathol & Mol Med, Hamilton, ON L8N 3Z5, Canada

[2] McGill Univ, Mol Oncol Grp, Lady Davis Inst Med Res, Montreal, PQ H3T 1E2, Canada

[3] McGill Univ, Dept Microbiol & Immunol, Montreal, PQ H3T 1E2, Canada

来源：

VACCINE | 2003年 / 21卷 / 13-14期

基金：

加拿大健康研究院;

关键词：

plasmid vaccine; interferon-regulatory factor;

D O I：

10.1016/S0264-410X(02)00694-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have been investigating the adjuvant properties of two super-activated interferon-regulatory factors (IRFs), IRF-3(5D) and IRF7/3A, identified in our previous studies of structure-function relationships, for enhancing plasmid vaccines. Intramuscular injection of plasmid cocktails encoding IRF-3(5D) and IRF7/3A molecules elicited cytotoxic T cell responses in over 80% of mice following a single immunization compared to a 20% response-rate using a control cocktail. Most interestingly, greater than 60% of mice immunized with the super-activated IRFs developed antigen-specific antibodies compared to 0% of the mice in the control group. Finally, vaccines which incorporated the super-activated IRFs provided greater protection against challenge with a recombinant vaccinia virus. These results support further investigation of the potential of these agents as adjuvants for genetic immunization. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：1363 / 1370

页数：8

共 23 条

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