CD4+ T regulatory cells from the colonic lamina propria of normal mice inhibit proliferation of enterobacteria-reactive, disease-inducing Th1-cells from scid mice with colitis

被引:26
作者
Gad, M [1 ]
Brimnes, I [1 ]
Claesson, MH [1 ]
机构
[1] Univ Copenhagen, Panum Inst, Dept Med Anat A, DK-2200 Copenhagen N, Denmark
关键词
scid mouse; colitis; inflammatory bowel disease; regulatory T cells; enteric bacteria;
D O I
10.1046/j.1365-2249.2003.02049.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Adoptive transfer of CD4(+) T cells into scid mice leads to a chronic colitis in the recipients. The transferred CD4(+) T cells accumulate in the intestinal lamina propria (LP), express an activated Th1 phenotype and proliferate vigously when exposed ex vivo to enteric bacterial antigens. As LP CD4(+) T cells from normal BALB/c mice do not respond to enteric bacterial antigens, we have investigated whether colonic LP-derived CD4(+) T cells from normal mice suppress the antibacterial response of CD4(+) T cells from scid mice with colitis. LP-derived CD4(+) T cells cocultured with bone marrow-derived dendritic cells effectively suppress the antibacterial proliferative response of CD4(+) T cells from scid mice with colitis. The majority of these LP T-reg cells display a nonactivated phenotype and suppression is independent of antigen exposure, is partly mediated by soluble factor(s) different from IL-10 and TGF-beta, and is not prevented by the addition of high doses of IL-2 to the assay culture. Functionally and phenotypically the T-reg cells of the present study differ from previously described subsets of T-reg cells. The presence of T cells with a regulatory potential in the normal colonic mucosa suggests a role for these cells in the maintenance of local immune homeostasis of the gut.
引用
收藏
页码:34 / 40
页数:7
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