Cloning of the gene coding for Staphylococcus intermedius exfoliative toxin and its expression in Escherichia coli

被引：19

作者：

Terauchi, R

Sato, H ^{[1
]}

Endo, Y

Aizawa, C

Maehara, N

机构：

[1] Kitasato Univ, Sch Vet Med & Anim Sci, Dept Vet Microbiol, Towada, Aomori 0348628, Japan

[2] Kitasato Inst, Minato Ku, Tokyo 1080072, Japan

来源：

VETERINARY MICROBIOLOGY | 2003年 / 94卷 / 01期

关键词：

Staphylococcus intermedius; Escherichia coli; gene; toxins;

D O I：

10.1016/S0378-1135(03)00047-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

An exfoliative toxin (SIET)-producing strain (D-52) of Staphylococcus intermedius derived from canine pyoderma did not possess large plasmids. Therefore, the gene coding for SIET was considered to be located on the chromosomal DNA. The SIET gene was cloned from the chromosomal DNA of S. intermedius and was expressed in Escherichia coli. The nucleotide sequence of the SIET gene consists of a coding region of 990 bp specifying a polypeptide of 330 amino acid residues, which included a putative 42-residue signal sequence. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：31 / 38

页数：8

共 27 条

[1]

CHARACTERIZATION OF STAPHYLOCOCCUS-INTERMEDIUS ISOLATES FROM CANINE PYODERMA AND FROM HEALTHY CARRIERS BY SDS-PAGE OF EXOPROTEINS, IMMUNOBLOTTING AND RESTRICTION-ENDONUCLEASE DIGEST ANALYSIS [J].