Expression in cereal plants of genes that inactivate fusarium mycotoxins

被引：22

作者：

Higa, A

Kimura, M

Mimori, K

Ochiai-Fukuda, T

Tokai, T

Takahashi-Ando, N

Nishiuchi, T

Igawa, T

Fujimura, M

Hamamoto, H

Usami, R

Yamaguchi, I

机构：

[1] RIKEN, Plant Sci Ctr, Lab Remediat Res, Wako, Saitama 3510198, Japan

[2] Toyo Univ, Dept Engn, Kawagoe, Saitama 3508585, Japan

[3] Toyo Univ, Dept Life Sci, Itakura, Gunma 3740193, Japan

[4] Kanazawa Univ, Inst Gene Res, Kanazawa, Ishikawa 9200934, Japan

[5] RIKEN, Plant Sci Ctr, Lab Adaptat & Resistance, Yokohama, Kanagawa 2300045, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2003年 / 67卷 / 04期

关键词：

fusarium head blight; mycotoxin and phytotoxin; transgenic cereals; trichothecene resistance; zearalenone detoxification;

D O I：

10.1271/bbb.67.914

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Trichothecene 3-O-acetyltransferase (encoded by Tri101) inactivates the virulence factor of the cereal pathogen Fusarium graminearum. Zearalenone hydrolase (encoded by zhd101) detoxifies the oestrogenic mycotoxin produced by the same pathogen. These genes were introduced into a model monocotyledon rice plant to evaluate their usefulness for decontamination of mycotoxins. The strong and constitutive rice Act1 promoter did not cause accumulation of TRI101 protein in transgenic rice plants. In contrast, the same promoter was suitable for transgenic production of ZHD101 protein; so far, five promising To plants have been generated. Low transgenic expression of Tri101 was suggested to be increased by addition of an Omega enhancer sequence upstream of the start codon.

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页码：914 / 918

页数：5

相关论文

共 14 条

[1] TRICHOTHECENE BIOSYNTHESIS IN FUSARIUM SPECIES - CHEMISTRY, GENETICS, AND SIGNIFICANCE [J].

DESJARDINS, AE ;

HOHN, TM ;

MCCORMICK, SP .

MICROBIOLOGICAL REVIEWS, 1993, 57 (03) :595-604

[2] The mystery of the trichothecene 3-O-acetyltransferase gene -: Analysis of the region around Tri101 and characterization of its homologue from Fusarium sporotrichioides [J].

Kimura, M ;

Matsumoto, G ;

Shingu, Y ;

Yoneyama, K ;

Yamaguchi, I .

FEBS LETTERS, 1998, 435 (2-3) :163-168

[3] Trichothecene 3-O-acetyltransferase protects both the producing organism and transformed yeast from related mycotoxins -: Cloning and characterization of Tri101 [J].

Kimura, M ;

Kaneko, I ;

Komiyama, M ;

Takatsuki, A ;

Koshino, H ;

Yoneyama, K ;

Yamaguchi, I .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (03) :1654-1661

[4] Features of Tri101, the trichothecene 3-O-acetyltransferase gene, related to the self-defense mechanism in Fusarium graminearum [J].

Kimura, M ;

Shingu, Y ;

Yoneyama, K ;

Yamaguchi, I .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1998, 62 (05) :1033-1036

[5] Microbial toxins in plant-pathogen interactions: Biosynthesis, resistance mechanisms, and significance [J].

Kimura, M ;

Anzai, H ;

Yamaguchi, I .

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2001, 47 (04) :149-160

[6] Isolation and characterization of novel cDNA clones of acidic chitinases and β-1,3-glucanases from wheat spikes infected by Fusarium graminearum [J].

Li, WL ;

Faris, JD ;

Muthukrishnan, S ;

Liu, DJ ;

Chen, PD ;

Gill, BS .

THEORETICAL AND APPLIED GENETICS, 2001, 102 (2-3) :353-362

[7] CONSTRUCTION OF EXPRESSION VECTORS BASED ON THE RICE ACTIN-1 (ACT1) 5' REGION FOR USE IN MONOCOT TRANSFORMATION [J].

MCELROY, D ;

BLOWERS, AD ;

JENES, B ;

WU, R .

MOLECULAR & GENERAL GENETICS, 1991, 231 (01) :150-160

[8] Transgenic expression of the TRI101 or PDR5 gene increases resistance of tobacco to the phytotoxic effects of the trichothecene 4,15-diacetoxyscirpenol [J].

Muhitch, MJ ;

McCormick, SP ;

Alexander, NJ ;

Hohn, TM .

PLANT SCIENCE, 2000, 157 (02) :201-207

[9]

OKUBARA PA, 2001, 2001 NAT FUS HEAD BL, P23

[10] Improved biomass productivity and water use efficiency under water deficit conditions in transgenic wheat constitutively expressing the barley HVA1 gene [J].

Sivamani, E ;

Bahieldin, A ;

Wraith, JM ;

Al-Niemi, T ;

Dyer, WE ;

Ho, THD ;

Qu, RD .

PLANT SCIENCE, 2000, 155 (01) :1-9