Expression in cereal plants of genes that inactivate fusarium mycotoxins

被引：22

作者：

Higa, A

Kimura, M

Mimori, K

Ochiai-Fukuda, T

Tokai, T

Takahashi-Ando, N

Nishiuchi, T

Igawa, T

Fujimura, M

Hamamoto, H

Usami, R

Yamaguchi, I

机构：

[1] RIKEN, Plant Sci Ctr, Lab Remediat Res, Wako, Saitama 3510198, Japan

[2] Toyo Univ, Dept Engn, Kawagoe, Saitama 3508585, Japan

[3] Toyo Univ, Dept Life Sci, Itakura, Gunma 3740193, Japan

[4] Kanazawa Univ, Inst Gene Res, Kanazawa, Ishikawa 9200934, Japan

[5] RIKEN, Plant Sci Ctr, Lab Adaptat & Resistance, Yokohama, Kanagawa 2300045, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2003年 / 67卷 / 04期

关键词：

fusarium head blight; mycotoxin and phytotoxin; transgenic cereals; trichothecene resistance; zearalenone detoxification;

D O I：

10.1271/bbb.67.914

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Trichothecene 3-O-acetyltransferase (encoded by Tri101) inactivates the virulence factor of the cereal pathogen Fusarium graminearum. Zearalenone hydrolase (encoded by zhd101) detoxifies the oestrogenic mycotoxin produced by the same pathogen. These genes were introduced into a model monocotyledon rice plant to evaluate their usefulness for decontamination of mycotoxins. The strong and constitutive rice Act1 promoter did not cause accumulation of TRI101 protein in transgenic rice plants. In contrast, the same promoter was suitable for transgenic production of ZHD101 protein; so far, five promising To plants have been generated. Low transgenic expression of Tri101 was suggested to be increased by addition of an Omega enhancer sequence upstream of the start codon.

引用

页码：914 / 918

页数：5

共 14 条

[11] A novel lactonohydrolase responsible for the detoxification of zearalenone: enzyme purification and gene cloning [J].