Analysis of parishin, parishin B and parishin C in Gastrodiae rhizoma by micellar electrokinetic capillary chromatography

被引:10
作者
Ku, YR [1 ]
Lin, YT [1 ]
Wen, KC [1 ]
Lin, JH [1 ]
Liao, CH [1 ]
机构
[1] Execut Yuan, Dept Hlth, Natl Labs Foods & Drugs, Taipei, Taiwan
关键词
pharmaceutical analysis; parishins;
D O I
10.1016/S0021-9673(98)00036-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
To evaluate the quality of Gastrodiae Rhizoma, a simple, rapid and accurate micellar electrokinetic capillary chromatographic method was developed for the assay of three marker constituents - parishin, parishin B and parishin C. The electrolyte was a buffer solution containing 100 mM sodium cholate and 20 mM sodium dihydrogenphosphate adjusted to pH 6.5 with 20 mM sodium tetraborate. Methylparaben was used as an internal standard and detected at 222 nm. The effects of buffer pH, electrolyte, temperature and voltage on separation are discussed. Regression equations revealed linear relationships (correlation coefficients: 0.9992-0.9998) between the peak-area rations of each marker to methylparaben. The relative standard deviations of three marker constituents ranged between 1.23-2.84% (intra-day) and 1.72-3.24% (inter-day). (C) 1998 Elsevier Science B.V.
引用
收藏
页码:330 / 336
页数:7
相关论文
共 7 条
[1]   DETERMINATION OF SOME DRUGS BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY - THE PSEUDO-EFFECTIVE MOBILITY AS PARAMETER FOR SCREENING [J].
ACKERMANS, MT ;
EVERAERTS, FM ;
BECKERS, JL .
JOURNAL OF CHROMATOGRAPHY, 1991, 585 (01) :123-131
[2]  
Chang H. M, 1986, PHARM APPL CHINESE M, VI, P185
[3]  
KU YR, 1995, J FOOD DRUG ANAL, V3, P233
[4]  
Lin JH, 1996, PHYTOCHEMISTRY, V42, P549, DOI 10.1016/0031-9422(95)00955-8
[5]  
LIN YM, 1993, J CHROMATOGR, V639, P323
[6]  
LIN YM, 1993, J CHROMATOGR, V634, P329
[7]  
LIN YM, 1994, ANAL CHIM ACTA, V288, P211