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Automated amino acid determination by high-performance liquid chromatography with 2-(9-anthryl)ethyl chloroformate as precolumn reagent

被引：8
作者：
Björklund, J
Einarsson, S
Engström, A
Grzegorczyk, A
Becker, HD
Josefsson, B [1 ]
机构：
[1] Univ Stockholm, Dept Analyt Chem, Arrhenius Labs Nat Sci, S-10691 Stockholm, Sweden
[2] Havsforskingsinst, IS-121 Reykjavik, Iceland
[3] Pharmacia & Upjohn Inc, S-11287 Stockholm, Sweden
[4] Sahlgrens Univ Hosp, Dept Clin Chem, S-41345 Gothenburg, Sweden
[5] Chalmers Univ Technol, Dept Phys Chem, S-41296 Gothenburg, Sweden
来源：
JOURNAL OF CHROMATOGRAPHY A | 1998年 / 798卷 / 1-2期
关键词：
derivatization; LC; anthrylethyl chloroformate; amino acids;
D O I：
10.1016/S0021-9673(97)00987-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A fully automated HPLC method for the determination of amino acids in protein hydrolysates has been developed, using 2-(9-anthryl)ethyl chloroformate and precolumn derivatization. This UV sensitive reagent reacts rapidly with both primary and secondary amines at room temperature to form stable carbamate derivatives. The reaction conditions have been thoroughly investigated. A complete separation of protein hydrolysates is accomplished within 40 min. The results an compared to those of the well known 9-fluorenylmethyl chloroformate method. Detection limits for UV and fluorescence detection were found to be 0.5 pmol and 0.06 pmol, respectively, (C) 1998 Elsevier Science B.V.
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页码：1 / 8
页数：8
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