Isotope dilution GC/MS determination of anandamide and other fatty acylethanolamides in rat blood plasma

被引:92
作者
Giuffrida, A [1 ]
Piomelli, D [1 ]
机构
[1] Inst Neurosci, San Diego, CA 92121 USA
关键词
anandamide; fatty acylethanolamide; cannabinoid; plasma; gas chromatography mass spectrometry;
D O I
10.1016/S0014-5793(98)00046-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Anandamide and allied fatty acylethanolamides (AEs) may act as signalling molecules in brain and peripheral tissues, In the present study, me describe an electron-impact gas chromatography/mass spectrometry (GC/MS) method based on isotope dilution, which may be used for the identification and quantification of anandamide and other AEs in biological matrices, The calibration curves for standard AEs were linear over the range 0-1000 pmol (r(2) = 0.99) with a coefficient of variation of 4% at 2.5 pmol. Detection and quantification limits mere in the high fmol to low pmol range for all AEs, Using this method we measured nanomolar concentrations of three endogenous AEs in deproteinated rat blood plasma (anandamide: 5.2 pmol/ml; palmitylethanolamide: 16.7 pmol/ml; oleylethanolamide: 8.1 pmol/ml). These results are consistent with a regulatory role of anandamide and other AEs in peripheral tissues. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码:373 / 376
页数:4
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