Rapid coupling of Surface Plasmon Resonance (SPR and SPRi) and ProteinChip™ based mass spectrometry for the identification of proteins in nucleoprotein interactions

被引：50

作者：

Bouffartigues, Emeline

Leh, Herve

Anger-Leroy, Marielle

Rimsky, Sylvie

Buckle, Malcolm

机构：

[1] Ecole Normale Super, Inst Alembert, CNRS UMR 8113, Lab Biotechnol & Pharmacol Genet Appliquee, F-91235 Cachan, France

[2] GenOpt SA Ctr Sci, F-91403 Orsay, France

来源：

NUCLEIC ACIDS RESEARCH | 2007年 / 35卷 / 06期

关键词：

D O I：

10.1093/nar/gkm030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We compared coupling approaches of SPR to LC-MS and ProteinChip (TM)-based mass spectrometry (SELDI (TM)) as a means of identifying proteins captured on DNA surfaces. The approach we outline has the potential to allow multiple, quantitative analysis of macromolecular interactions followed by rapid mass spectrometry identification of retained material.

引用

页数：10

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