Somatic hybrids between navel orange (Citrus sinensis) and grapefruit (C-paradisi) for seedless triploid breeding

被引:26
作者
Guo, WW [1 ]
Deng, XX [1 ]
Yi, HL [1 ]
机构
[1] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
基金
中国国家自然科学基金;
关键词
Citrus; early flowering; electrofusion; RAPD; somatic hybrids;
D O I
10.1023/A:1004031621777
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Protoplasts from cell suspension cultures of 'Bonnaza' navel orange ( Citrus sinensis (L.) Osbeck) were electrically fused with mesophyll protoplasts isolated from seedless 'Red Blush' grapefruit (Citrus paradisi). After 6 months of culture, a total of 20 plants were regenerated. Root tip chromosome counting revealed that 4 of them were tetraploids (2n = 4x = 36) and the rest were diploids (2n = 2x = 18) morphologically resembling the mesophyll parent. After 6 months of transplantation into the greenhouse, 4 of the diploid mesophyll regenerants unexpectedly flowered, but this phenomenon disappeared in the next year. This is the first report of precocious flowering in citrus via protoplast fusion. RAPD analysis further confirmed that the tetraploid regenerants were somatic hybrids while the diploid regenerants were mesophyll parent type. This somatic hybrid will be utilized as a possible pollen parent for improving the seedy pummelo cultivars in China by producing triploid seedless pummelo hybrid. The mechanism of early flowering was also discussed.
引用
收藏
页码:281 / 285
页数:5
相关论文
共 36 条
[1]  
Deng XiuXin, 2000, Acta Horticulturae Sinica, V27, P1
[2]  
DENG XX, 1992, SCI HORTIC-AMSTERDAM, V49, P55, DOI 10.1016/0304-4238(92)90142-Y
[3]   PRODUCTION AND CHARACTERIZATION OF SOMATIC HYBRIDS BETWEEN SOLANUM-MELONGENA-L AND SOLANUM-SISYMBRIIFOLIUM LAM [J].
GLEDDIE, S ;
KELLER, WA ;
SETTERFIELD, G .
THEORETICAL AND APPLIED GENETICS, 1986, 71 (04) :613-621
[4]   EMBRYOGENESIS INVITRO AND NONCHIMERIC TETRAPLOID PLANT-RECOVERY FROM UNDEVELOPED CITRUS OVULES TREATED WITH COLCHICINE [J].
GMITTER, FG ;
LING, XB .
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 1991, 116 (02) :317-321
[5]   COLCHICINE-INDUCED POLYPLOIDY IN CITRUS EMBRYOGENIC CULTURES, SOMATIC EMBRYOS, AND REGENERATED PLANTLETS [J].
GMITTER, FG ;
LING, XB ;
CAI, CY ;
GROSSER, JW .
PLANT SCIENCE, 1991, 74 (01) :135-141
[6]  
Grosser J. W., 1990, Plant Breeding Reviews, V8, P339
[7]  
Grosser JW, 1996, THEOR APPL GENET, V92, P577, DOI 10.1007/BF00224561
[8]   Further evidence of a cybridization requirement for plant regeneration from citrus leaf protoplasts following somatic fusion [J].
Grosser, JW ;
Gmitter, FG ;
Tusa, N ;
Recupero, GR ;
Cucinotta, P .
PLANT CELL REPORTS, 1996, 15 (09) :672-676
[9]  
GROSSER JW, 1996, P INT SOC CITR, P1246
[10]  
GROSSER JW, 1996, THEOR APPL GENET, P1246