The influence of solvent formulations on thermosensitive hydroxybutyl chitosan hydrogel as a potential delivery matrix for cell therapy

被引:35
作者
Bao, Zixian [1 ]
Jiang, Changqing [2 ]
Wang, Zhiguo [3 ]
Ji, Qiuxia [3 ]
Sun, Guohui [1 ]
Bi, Shichao [1 ]
Liu, Ya [1 ]
Chen, Xiguang [1 ]
机构
[1] Ocean Univ China, Coll Marine Life Sci, Yushan Rd, Qingdao 266003, Shandong, Peoples R China
[2] Qingdao Municipal Hosp, Qingdao 266003, Shandong, Peoples R China
[3] Qingdao Univ, Coll Med, Affiliated Hosp, Dept Plast Surg, Qingdao 266013, Shandong, Peoples R China
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
Hydroxybutyl chitosan; Thermosensitive hydrogel; Solvent formulation; Cell delivery; PROLIFERATION; SCAFFOLDS; CULTURE; MICE; PH;
D O I
10.1016/j.carbpol.2017.04.038
中图分类号
O69 [应用化学];
学科分类号
070301 [无机化学];
摘要
Many cell delivery matrices have been developed due to the low transplantation efficiency of cell,therapy. In the present study, thermos ensitive hydroxybutyl chitosan (HBC) hydrogels were prepared with different formulations' solvent (Dulbecco's modified eagle's medium/phosphate buffered saline [DMEM/PBS], 0:100, 30:70, 50:50, 70:30,100:0 [v/v]). Their gelation temperature was raised with DMEM ratio increase (from 9.5 degrees C to 20.9 degrees C). Pore sizes of HBC hydrogels treated with high ionic strength solutions became smaller. HUVECs cultured with HBC hydrogels exhibited proliferation in high DMEM ratio groups, and the optimal solvent formulation was DMEM/PBS 70:30 (v/v). Upon exposure to PBS, HUVECs encapsulated in HBC hydrogels could survive better than that when seeded on the surface of HBC hydrogels. These results demonstrated that HBC hydrogel could be a potential cell delivery matrix for cell therapy applications, especially when DMEM proportion in solvent formulations was higher than 50% (DMEM/PBS > 50:50 [v/v]). (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码:80 / 88
页数:9
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