Immunohistochemical localization of histamine H3 receptors in rodent skin, dorsal root ganglia, superior cervical ganglia, and spinal cord:: Potential antinociceptive targets

被引:70
作者
Cannon, Keri E.
Chazot, Paul L.
Hann, Victoria
Shenton, Fiona
Hough, Lindsay B.
Rice, Frank L.
机构
[1] Albany Med Coll, Ctr Neuropharmacol & Neurosci, Albany, NY 12208 USA
[2] Univ Durham, Sch Biol & Biomed Sci, Durham, England
基金
英国惠康基金;
关键词
histamine; H-3; receptors; arterial innervation; A delta fibers; a beta fibers; calcitonin gene-related peptide; substance P; sensory innervation;
D O I
10.1016/j.pain.2006.09.039
中图分类号
R614 [麻醉学];
学科分类号
100217 ;
摘要
Activation of histamine H-3 receptors (H(3)Rs) reduces inflammation and nociception, but the existence of H(3)Rs on peripheral innervation has never been demonstrated. Here we use antibodies to locate H3Rs in whisker pads, hairy and glabrous hind paw skin, dorsal root ganglia (DRGs), and spinal cords of rats, wild type mice, and H3R knockout (H3KO) mice. Although H3Rs have been hypothesized to be on C and sympathetic fibers, H3R-like immunoreactivity (H3R-LI) was only detected on presumptive periarterial A delta fibers and on A beta fibers that terminated in Meissner's corpuscles and as lanceolate endings around hair follicles. The H3R-positive periarterial fibers were thin-caliber and coexpressed immunoreactivity for calcitonin gene-related peptide (CGRP), substance P, acid sensing ion channel 3, and 200 kDa neurofilament protein (NF). H3R-LI was also detected on epidermal keratinocytes and Merkel cells, but not on Merkel endings, C fibers, any other A delta fibers, or sympathetic fibers. In DRGs, H3R-LI was preponderantly on medium to large neurons coexpressing NF-LI and mostly CGRP-LI. In dorsal horn, CGRP-positive fibers with and without H3R-LI ramified extensively in lamina II; many of the former formed a plexus in lamina V. Low levels of H3R-LI were also present on A beta fibers penetrating superficial and into deep er laminae. The distribution of H3R-LI was similar in rats and wild type mice, but was eliminated or strongly reduced in A delta fibers and A beta fibers, respectively, in H3KO mice. Taken with recently published behavioral results, the present findings suggest that periarterial, peptidergic, H3R-containing A delta fibers may be sources of high threshold mechanical nociception. (c) 2006 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:76 / 92
页数:17
相关论文
共 76 条
[1]   ESTIMATION OF NUCLEAR POPULATION FROM MICROTOME SECTIONS [J].
ABERCROMBIE, M .
ANATOMICAL RECORD, 1946, 94 (02) :239-247
[2]   AUTO-INHIBITION OF BRAIN HISTAMINE-RELEASE MEDIATED BY A NOVEL CLASS (H-3) OF HISTAMINE-RECEPTOR [J].
ARRANG, JM ;
GARBARG, M ;
SCHWARTZ, JC .
NATURE, 1983, 302 (5911) :832-837
[3]   HIGHLY POTENT AND SELECTIVE LIGANDS FOR HISTAMINE RECEPTORS-H-3 [J].
ARRANG, JM ;
GARBARG, M ;
LANCELOT, JC ;
LECOMTE, JM ;
POLLARD, H ;
ROBBA, M ;
SCHUNACK, W ;
SCHWARTZ, JC .
NATURE, 1987, 327 (6118) :117-123
[4]   ENHANCEMENT OF PHAGOCYTOSIS - A NEWLY FOUND ACTIVITY OF SUBSTANCE-P RESIDING IN ITS N-TERMINAL TETRAPEPTIDE SEQUENCE [J].
BARSHAVIT, Z ;
GOLDMAN, R ;
STABINSKY, Y ;
GOTTLIEB, P ;
FRIDKIN, M ;
TEICHBERG, VI ;
BLUMBERG, S .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1980, 94 (04) :1445-1451
[5]   NERVOUS OUTFLOW FROM CATS FOOT DURING NOXIOUS RADIANT HEAT STIMULATION [J].
BECK, PW ;
HANDWERKER, HO ;
ZIMMERMANN, M .
BRAIN RESEARCH, 1974, 67 (03) :373-386
[6]   INTERACTIONS BETWEEN THE TACHYKININS AND CALCITONIN GENE-RELATED PEPTIDE LEAD TO THE MODULATION OF EDEMA FORMATION AND BLOOD-FLOW IN RAT SKIN [J].
BRAIN, SD ;
WILLIAMS, TJ .
BRITISH JOURNAL OF PHARMACOLOGY, 1989, 97 (01) :77-82
[7]   EVIDENCE THAT CALCITONIN GENE RELATED PEPTIDE CONTRIBUTES TO INFLAMMATION IN THE SKIN AND JOINT [J].
BRAIN, SD ;
CAMBRIDGE, H ;
HUGHES, SR ;
WILSONCROFT, P .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES-SERIES, 1992, 657 :412-419
[8]   MYELINATED AFFERENT FIBRES RESPONDING SPECIFICALLY TO NOXIOUS STIMULATION OF SKIN [J].
BURGESS, PR ;
PERL, ER .
JOURNAL OF PHYSIOLOGY-LONDON, 1967, 190 (03) :541-&
[9]   Inhibition of chemical and low-intensity mechanical nociception by activation of histamine H3 receptors [J].
Cannon, KE ;
Hough, LB .
JOURNAL OF PAIN, 2005, 6 (03) :193-200
[10]   Activation of spinal histamine H3 receptors inhibits mechanical nociception [J].
Cannon, KE ;
Nalwalk, JW ;
Stadel, R ;
Ge, P ;
Lawson, D ;
Silos-Santiago, I ;
Hough, LB .
EUROPEAN JOURNAL OF PHARMACOLOGY, 2003, 470 (03) :139-147