A nucleoside that contains a rigid nitroxide spin label: A fluorophore in disguise

被引：114

作者：

Barhate, Nivrutti

Cekan, Pavol

Massey, Archna P.

Sigurdsson, Snorri Th.

机构：

[1] Univ Iceland, Inst Sci, IS-107 Reykjavik, Iceland

[2] Univ Washington, Dept Chem, Seattle, WA 98195 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 15期

关键词：

ePR spectroscopy; fluorescence spectroscopy; nucleic acids; site-directed spin labeling (SDSL); spin labels;

D O I：

10.1002/anie.200603993

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Take a spin: The nucleoside Ç (C-spin), which contains a rigid nitroxide spin label, allows the structure and dynamics of nucleic acids into which it is incorporated to be studied by EPR spectroscopy (left spectrum). Reduction of the nitroxide with a mild reducing agent renders the nucleoside fluorescent (right spectrum). Thus, the same nucleic acid can be studied with two complementary spectroscopic techniques. (Chemical Equation Presented). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：2655 / 2658

页数：4

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